protein

RECOMBINANT MOUSE MCP-1

MBS232337

0.01 mg

430 USD

Recombinant Protein

RECOMBINANT MOUSE MCP-1

MCP-1

C-C motif chemokine 2; C-C motif chemokine 2; C-C motif chemokine 2; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic protein 1; platelet-derived growth factor-inducible protein JE; small inducible cytokine A2; small-inducible cytokine A2; chemokine (C-C motif) ligand 2; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; MCP-1; Platelet-derived growth factor-inducible protein JE; Small-inducible cytokine A2

MCP-1

Ccl2; Ccl2; JE; HC11; MCAF; MCP1; MCP-1; Scya2; Sigje; SMC-CF; AI323594; Je; Mcp1; Scya2; MCP-1

CCL2_MOUSE

148

>98% by SDS PAGE and HPLC analysis

Purified recombinant protein - lyophilised

Approximate Protein Concentration: 0.1mg/ml after reconstitution

Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 3 months from date of reconstitution.

ELISA (EIA), Functional Assays (FN), Western Blot (WB)

ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;. Functional Assays: Minimum Dilution: 1.0; Maximum Dilution: 20ng/ml;. Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane

Reconstitution: Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. Preparation: Recombinant protein prepared from E Coli.

Target Species: Mouse

Recombinant mouse MCP-1 is 13.8 kDa peptide corresponding to full-length mature mouse monocyte chemotactic protein 1. MCP-1, otherwise known as CCL2 or JE, is a secreted chemokine involved in the recruitment of monocytes to sites of injury or inflammation (Lu, B. et al., 1998).

6755430

NP_035463.1

NM_011333.3

P10148

Protein Molecular Weight: 13.8kD (125 amino acid sequence)

Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Herpes Simplex Infection Pathway (377874); Herpes Simplex Infection Pathway (377865); Influenza A Pathway (217174)

This gene is one of several cytokine genes clustered on chromosome 11. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and memory T cells but not for neutrophils. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis. [provided by RefSeq, Sep 2015]

CCL13: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. By IL1/interleukin-1 and TNF. Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. Belongs to the intercrine beta (chemokine CC) family. Protein type: Secreted; Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide. Cellular Component: extracellular space; cell soma; rough endoplasmic reticulum; endocytic vesicle; perinuclear region of cytoplasm; cytoplasm; dendrite; extracellular region; synapse; perikaryon; nerve terminal. Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; chemokine activity; CCR2 chemokine receptor binding; cytokine activity. Biological Process: positive regulation of cell adhesion; positive regulation of leukocyte migration; positive regulation of collagen biosynthetic process; chemotaxis; positive regulation of synaptic transmission; positive regulation of cellular extravasation; regulation of cell shape; transforming growth factor beta receptor signaling pathway; response to wounding; positive regulation of cell-cell adhesion; angiogenesis; inflammatory response; lymphocyte chemotaxis; neutrophil chemotaxis; cytokine and chemokine mediated signaling pathway; cytoskeleton organization and biogenesis; positive regulation of tumor necrosis factor production; glial cell migration; macrophage chemotaxis; leukocyte migration during inflammatory response; cellular calcium ion homeostasis; positive regulation of leukocyte mediated cytotoxicity; negative regulation of angiogenesis; response to heat; eosinophil chemotaxis; positive regulation of endothelial cell proliferation; immune response; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; positive regulation of nitric-oxide synthase biosynthetic process

0.01 mg

430 USD