RECOMBINANT HUMAN TGF ALPHA | MyBioSource MBS232239 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

RECOMBINANT HUMAN TGF ALPHA

catalog :

MBS232239

quantity :

0.1 mg

price :

430 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS232239

products type :

Recombinant Protein

products full name :

RECOMBINANT HUMAN TGF ALPHA

products short name :

TGF ALPHA

other names :

protransforming growth factor alpha isoform 2 preproprotein; Protransforming growth factor alpha; protransforming growth factor alpha; TGF-alpha; transforming growth factor, alpha; EGF-like TGF; ETGF; TGF type 1

products gene name :

TGF ALPHA

other gene names :

TGFA; TGFA; TFGA; TGF-alpha; ETGF

uniprot entry name :

TGFA_HUMAN

reactivity :

Mouse

sequence length :

159

purity :

>98% by SDS PAGE and HPLC

form :

Recombinant protein - lyophilised

concentration :

Approximate Protein Concentration: 1.0 mg/ml after reconstitution

storage stability :

Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 3 months from date of reconstitution.

tested application :

ELISA (EIA), Functional Assays (FN), Western Blot (WB)

app notes :

ELISA: Recombinant human TGFalpha may be used as a standard for ELISA applications with either a purifed human TGFalpha antibody or a biotinylated human TGFalpha antibody. Western Blot: Recombinant human TGFalpha may be used as the positive control for Western Blotting applications with either a purified human TGFalpha antibody or a biotinylated human TGFalpha antibody. ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well. Functional Assays: Minimum Dilution: 0.5; Maximum Dilution: 25ng/ml. Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane

other info1 :

Reconstitution: Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For extended storage the addition of 0.1% bovine serum albumin (BSA) is recommended. Preparation: Recombinant protein expressed in E Coli.

other info2 :

Activity: >1.4 x 106 Units/mg. Target Species: Human

products description :

Recombinant human TGFalpha is a 5.5kDa peptide corresponding to full-length mature human transforming growth factor alpha. TGFalpha is an EGF-related growth factor that stimulates the proliferation of epidermal and epithelial cells.

ncbi gi num :

153791414

ncbi acc num :

NP_001093161.1

ncbi gb acc num :

NM_001099691.2

uniprot acc num :

P01135

ncbi mol weight :

Protein Molecular Weight: 5.5kD (50 Amino Acid Sequence)

ncbi pathways :

Direct P53 Effectors Pathway (137939); ErbB Receptor Signaling Network Pathway (138016); ErbB Signaling Pathway (198844); ErbB Signaling Pathway (83049); ErbB Signaling Pathway (458); FOXM1 Transcription Factor Network Pathway (137935); Glioma Pathway (83110); Glioma Pathway (522); Non-small Cell Lung Cancer Pathway (83119); Non-small Cell Lung Cancer Pathway (531)

ncbi summary :

This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

uniprot summary :

TGFalpha: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2. Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Ligand, receptor tyrosine kinase; Cell cycle regulation; Membrane protein, integral. Chromosomal Location of Human Ortholog: 2p13. Cellular Component: extracellular space; cell surface; basolateral plasma membrane; perinuclear region of cytoplasm; integral to plasma membrane; plasma membrane; cytoplasmic vesicle; nucleus. Molecular Function: protein binding; growth factor activity; glycoprotein binding; epidermal growth factor receptor binding. Biological Process: response to drug; epidermal growth factor receptor signaling pathway; cell proliferation; wound healing; positive regulation of epidermal growth factor receptor activity; positive regulation of mitosis; activation of MAPK activity; angiogenesis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis

size1 :

0.1 mg

price1 :

430 USD

more info or order :

MyBioSource product webpage