RECOMBINANT HUMAN ERYTHROPOIETIN ALPHA | MyBioSource MBS232058 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

RECOMBINANT HUMAN ERYTHROPOIETIN ALPHA

catalog :

MBS232058

quantity :

0.05 mg

price :

430 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS232058

products type :

Recombinant Protein

products full name :

RECOMBINANT HUMAN ERYTHROPOIETIN ALPHA

products short name :

ERYTHROPOIETIN ALPHA

other names :

erythropoietin; Erythropoietin; erythropoietin; epoetin; erythropoietin; INN: Epoetin

other gene names :

EPO; EPO; EP; MVCD2

uniprot entry name :

EPO_HUMAN

sequence length :

193

purity :

>98.0% by HPLC analysis and SDS-PAGE

form :

Recombinant protein - lyophilised

concentration :

Total protein concentration 0.2mg/ml after reconstitution

storage stability :

Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of reconstitution.

tested application :

Functional Assays (FN)

other info1 :

Reconstitution: Reconstitute with 250ul sterile deionised water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of a carrier protein (0.1% HSA or BSA) is recommended.

other info2 :

Buffer Solution: Citrate buffered saline. Activity: The specific activity was measured by normocythaemic mice and found to be 150,000 IU/mg. Target Species: Human

products description :

MBS232058 is recombinant human erythropoietein alpha, produced in CHO cells. It is a single polypeptide chain containing 166 amino acids with a predicted molecular mass of 21,000 Daltons and an apparent molecular mass of 30,400 Daltons.

ncbi gi num :

62240997

ncbi acc num :

NP_000790.2

ncbi gb acc num :

NM_000799.2

uniprot acc num :

P01588

ncbi mol weight :

21,307 Da

ncbi pathways :

Cellular Response To Hypoxia Pathway (645259); Cellular Responses To Stress Pathway (645258); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); EPO Receptor Signaling Pathway (198882); EPO Signaling Pathway (138051); HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); HIF-2-alpha Transcription Factor Network Pathway (137956); Hematopoietic Cell Lineage Pathway (83078)

ncbi summary :

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008]

uniprot summary :

EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 7q22. Cellular Component: extracellular space; cell surface; extracellular region. Molecular Function: protein binding; erythropoietin receptor binding; hormone activity; protein kinase activator activity. Biological Process: hemoglobin biosynthetic process; erythrocyte maturation; blood circulation; apoptosis; positive regulation of transcription, DNA-dependent; response to lipopolysaccharide; response to testosterone stimulus; signal transduction; response to salt stress; response to vitamin A; peptidyl-serine phosphorylation; response to hyperoxia; positive regulation of tyrosine phosphorylation of Stat5 protein; response to estrogen stimulus; positive regulation of protein kinase activity; positive regulation of cell proliferation; positive regulation of Ras protein signal transduction; response to axon injury; positive regulation of neuron differentiation; response to electrical stimulus; positive regulation of DNA replication; embryo implantation; aging. Disease: Microvascular Complications Of Diabetes, Susceptibility To, 2

size1 :

0.05 mg

price1 :

430 USD

more info or order :

MyBioSource product webpage