protein

NATIVE HUMAN LOW DENSITY LIPOPROTEIN (OXIDIZED)

MBS230182

2 mg

600 USD

Purified Protein

NATIVE HUMAN LOW DENSITY LIPOPROTEIN (OXIDIZED)

LOW DENSITY LIPOPROTEIN

low density lipoprotein, partial; Low density lipoprotein; Low density lipoprotein

LDLR

E1B4T0_HUMAN

76

Purified. Purified oxidised LDL from human plasma - liquid

Total protein concentration 2.0 mg/ml

Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Shelf Life: This product is stable for approximately 4 weeks when handled aseptically and stored at 4 degree C.

Functional Assays (FN)

Perservative Stabilisers: 0.3 mM EDTA. Preparation: LDL is purified to homogeneity by ultra-centrifugation. Oxidised using 20 uM cupric sulphate in PBS at 37 degree C for 24 hours. Oxidation is terminated with excess EDTA. The level of oxidation is measured using TBARS (Thiobarbituric acid reactive substances) determination using a Malondialdehyde (MDA) standard. The level of oxidation is batch specific and available on request.

Buffer Solution: Phosphate buffered saline. Target Species: Human. Health and Safety Information: Donor material tested and found negative for HIV1, HIV2, HIV1 antigen, HBsAg, the antibody to HTLV1, HCV, HBcAg, ALT and syphilis. As no test can completely guarantee this material to be free of pathogens it should be handled as potentially infectious.

Low density lipoprotein (LDL) oxidative modification is one of the major processes involved in atherosclerosis along with other degenerative disorders. Oxidised LDL is preferentially accumulated by macrophages which become so called "foam cells" within the atheromatous plaque and contributes to the accumulation of cholesterol, typical of atherosclerosis.

306411097

ADM85894.1

8,219 Da

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, misc.; Cell surface. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: Golgi apparatus; cell surface; membrane; lysosome; integral to plasma membrane; late endosome; early endosome; plasma membrane; coated pit; endosome membrane; receptor complex; external side of plasma membrane. Molecular Function: low-density lipoprotein receptor activity; very-low-density lipoprotein receptor activity; protein binding; low-density lipoprotein binding; calcium ion binding; clathrin heavy chain binding; glycoprotein binding. Biological Process: receptor-mediated endocytosis; lipoprotein catabolic process; cholesterol metabolic process; phototransduction, visible light; cholesterol homeostasis; cholesterol transport; viral reproduction; cholesterol absorption; phospholipid transport; endocytosis; lipoprotein metabolic process; lipid metabolic process; retinoid metabolic process. Disease: Hypercholesterolemia, Familial

2 mg

600 USD