MOUSE ANTI PIG CD8 BETA CHAIN: FITC | MyBioSource MBS224819 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI PIG CD8 BETA CHAIN: FITC

catalog :

MBS224819

quantity :

0.1 mg

price :

505 USD

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

[PPT23]

reactivity :

human

application :

flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain:RPE

image 2 :

Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain:FITC (This product)

image 3 :

Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain followed by Goat anti Mouse IgG (H/L):FITC

product information

catalog number :

MBS224819

products type :

Antibody

products full name :

MOUSE ANTI PIG CD8 BETA CHAIN: FITC

products short name :

[CD8 BETA CHAIN]

other names :

[CD8 beta chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein CD8 beta chain; CD8b molecule; CD_antigen: CD8b]

products gene name :

[CD8]

other gene names :

[CD8B; CD8B; LY3; P37; LEU2; LYT3; CD8B1; CD8B1]

uniprot entry name :

CD8B_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[PPT23]

host :

Mouse

reactivity :

Pig

sequence length :

specificity :

CD8 BETA

form :

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

concentration :

IgG concentration 0.1 mg/ml

storage stability :

Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch

tested application :

Flow Cytometry (FC/FACS)

app notes :

Flow Cytometry: Neat- 1/10. Use 10ul of the suggested working dilution to label 1x10 6 cells in 100ul. Where the product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their system using appropriate negative/positive controls.

image1 heading :

Flow Cytometry (FC/FACS)

image2 heading :

Flow Cytometry (FC/FACS)

image3 heading :

Flow Cytometry (FC/FACS)

other info1 :

Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. Preservative Stabilisers: 0.09% Sodium Azide (NaN3), 1% Bovine Serum Albumin . Buffer Solution: Phosphate buffered saline

other info2 :

Immunogen: Porcine thymus membrane lysate. Fusion partners: Lymph node cells from immunized BALB/c mice were fused with cells of the NSO myeloma cell line

products description :

Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen. Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3 + , CD4 - , CD8 hi and as such defines this antibody as a specific marker of porcine alpha/beta T cells. Characterization of clone PPT23 has shown that in thymic tissue both CD8lo and CD8hi cells are recognized (Yang H & Parkhouse R.M. 1997). Inhibition studies have demonstrated that clone PPT23 recognizes a different epitope of on the CD8 beta chain to clone PPT22.

ncbi gi num :

4261577

ncbi acc num :

AAD13877.1

ncbi mol weight :

22,237 Da

ncbi pathways :

Adaptive Immune System Pathway (1269171); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Disease Pathway (1268854); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); HIV Infection Pathway (1269057); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489)

ncbi summary :

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010]

uniprot summary :

CD8B: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. 8 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 2p12. Cellular Component: early endosome membrane; external side of plasma membrane; extracellular region; integral to plasma membrane; plasma membrane; T cell receptor complex. Molecular Function: coreceptor activity; MHC class I protein binding; protein binding. Biological Process: adaptive immune response; immune response; regulation of defense response to virus by virus; regulation of immune response; T cell activation; transmembrane receptor protein tyrosine kinase signaling pathway; viral reproduction

size1 :

0.1 mg

price1 :

505 USD

more info or order :

MyBioSource product webpage