MOUSE ANTI HUMAN CD39: Low Endotoxin | MyBioSource MBS224810 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN CD39: Low Endotoxin

catalog :

MBS224810

quantity :

0.5 mg

price :

675 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[A1]

reactivity :

human

application :

immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Staining of human peripheral blood lymphocytes with Mouse anti Human CD39:Low Endotoxin followed by Rabbit F(ab )2 anti Mouse IgG:FITC

image 2 :

Staining of human peripheral blood lymphocytes with Mouse anti Human CD39 followed by Rabbit F(ab )2 anti Mouse IgG:FITC

image 3 :

Staining of human peripheral blood lymphocytes with Mouse anti Human CD39:FITC

product information

catalog number :

MBS224810

products type :

Antibody

products full name :

MOUSE ANTI HUMAN CD39: Low Endotoxin

products short name :

[CD39]

products name syn :

[CD39]

other names :

[Ectonucleoside triphosphate diphosphohydrolase 1; Ectonucleoside triphosphate diphosphohydrolase 1; ectonucleoside triphosphate diphosphohydrolase 1; ectonucleoside triphosphate diphosphohydrolase 1; Ecto-ATP diphosphohydrolase 1; Ecto-ATPDase 1; Ecto-ATPase 1; Ecto-apyrase; Lymphoid cell activation antigen; CD_antigen: CD39]

products gene name :

[CD39]

other gene names :

[ENTPD1; ENTPD1; CD39; SPG64; ATPDase; NTPDase-1; CD39; NTPDase 1; Ecto-ATPDase 1; Ecto-ATPase 1]

uniprot entry name :

ENTP1_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[A1]

host :

Mouse

reactivity :

Human

sequence length :

510

specificity :

CD39

form :

Low Endotoxin. Purified IgG - liquid

concentration :

IgG concentration 1.0 mg/ml

storage stability :

Store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Flow Cytometry (FC/FACS), Functional Assay, Immunofluorescence (IF)

app notes :

Suggested Dilution for Flow Cytometry: 1/10-1/50. Flow Cytometry: Use 10ul of the suggested working dilutionto label 10 6 cells or 100ul whole blood.

image1 heading :

Flow Cytometry (FC/FACS)

image2 heading :

Flow Cytometry (FC/FACS)

image3 heading :

Flow Cytometry (FC/FACS)

image4 heading :

Testing Data

image4 description :

Published customer image: Mouse anti human CD39, clone A1 used for the evaluation of CD39 expression on cultured mononuclear cells by flow cytometry.Image caption:Phenotype and functional analysis of Foxp3hi and Foxp3int T cells in direct co-cultures or separated through a transwell. T cells from direct or transwell-separated iRBC co-cultures were harvested on day 6 of culture and either re-stimulated with PMA/ionomycin for intracellular cytokine staining, or phenotyped directly. Foxp3hi:Foxp3int ratios and the percentage of IFN? or IL-4 producing cells within the CD25hiFoxp3int population were determined for 5 donors. Values for transwell-separated T cells (black bars) are expressed as fold change following normalization onto values in direct co-cultures (white bars) for each donor (Mean+/-SEM) and compared by paired Student s t-test (A). Freshly isolated CD25-Foxp3- and CD25+Foxp3+ CD4+ T cells (day 0) and CD4+CD25-Foxp3-, Foxp3int and Foxp3hi CD4+CD25hi T cells from direct or transwell separated cultures (day 6) from 3 donors were phenotyped for a wide range of markers, and expression levels for each surface marker within each population were normalized on isotype control staining, when analysed as median fluorescence intensity (MFI). Expression levels of surface markers on CD4+CD25-Foxp3- cells were comparable between conditions for each donor (data not shown). Thus, to compare the phenotype of day 0 CD25+Foxp3+ nTregs, and day 6 induced CD25hiFoxp3int and CD25hiFoxp3hi cells (black bars), expression levels are expressed as fold change (Mean+/-SEM) compared to CD4+CD25-Foxp3- cells (white bars, relative level = 1) for each donor and condition (B). Experiments were set up with a 2:1 iRBC:PBMC ratio.From: Scholzen A, Mittag D, Rogerson SJ, Cooke BM, Plebanski M (2009) Plasmodium falciparum -Mediated Induction of Human CD25hiFoxp3hi CD4 T Cells Is Independent of Direct TCR Stimulation and Requires IL-2, IL-10 and TGFbeta.PLoS Pathog 5(8): e1000543.

image5 heading :

Testing Data

image5 description :

Published customer image: Mouse anti human CD39, clone A1 used for the evaluation of CD39 expression on cultured mononuclear cells by flow cytometry.Image caption:Phenotype of Foxp3hi and Foxp3int CD4+CD25hi T cells induced by malaria-infected RBCs. After 8 days of iRBC:PBMC co-culture, immuno magnetic selection was used to isolate CD4+CD25hi (black line) and CD25- cells (grey filled). CD25 and Foxp3 expression in the two sorted populations was analysed by flow cytometry (A). mRNA levels for Foxp3, TGF 1 and IL-10 were normalized on 18SrRNA levels for each donor. Data from 8 donors (Mean+/-SEM) are shown, presented as relative mRNA levels normalized on mRNA levels in CD4+CD25- T cells for each donor (A). PBMC of 3 donors were phenotyped on day 0 prior to iRBC-PBMC co-culture set up, and after 6 days of iRBC:PBMC co-culture (2:1 ratio). Dot plots show the gating strategies for Foxp3 expressing cells on day 0 and day 6, respectively (B). Representative histograms depicting isotype control staining (grey filled) and specific staining for various surface markers on CD25-Foxp3- and CD25hi Foxp3-expressing CD4 T cells (black line) from each respective culture are shown (B).From: Scholzen A, Mittag D, Rogerson SJ, Cooke BM, Plebanski M (2009) Plasmodium falciparum -Mediated Induction of Human CD25hiFoxp3hi CD4 T Cells Is Independent of Direct TCR Stimulation and Requires IL-2, IL-10 and TGFbeta.PLoS Pathog 5(8): e1000543.

other info1 :

Target Species: Human. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Buffer Solution: Phosphate buffered saline

other info2 :

Endotoxin Level: <0.1 EU/ug. Immunogen: PHA activated human lymphocytes. Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.

products description :

Mouse anti Human CD39, clone A1 recognizes the human CD39 cell surface antigen, a ~70-100 kDa molecule expressed on peripheral blood B cells, T cells and monocytes, and weakly expressed by granulocytes. CD39 has intrinsic ecto-ATPase activity (Wang et al. 1996), and expression can be induced on T cells and increased on B cells, as a late activation antigen (Maliszewski et al. 1994). Mouse anti Human CD39, clone A1 has been shown to block MHC independent target cell recognition by hapten-specific CTL (Scholzen et al. 2009).

ncbi gi num :

1705710

ncbi acc num :

P49961.1

uniprot acc num :

P49961

ncbi mol weight :

59,325 Da

ncbi pathways :

Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (307); Pyrimidine Metabolism Pathway (82946); Pyrimidine Metabolism Pathway (309); UTP And CTP Dephosphorylation I Pathway (908000); UTP And CTP Dephosphorylation II Pathway (782399); UTP And CTP Dephosphorylation II Pathway (908002)

ncbi summary :

The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]

uniprot summary :

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well.

size1 :

0.5 mg

price1 :

675 USD

more info or order :

MyBioSource product webpage