RAT ANTI MOUSE CD16 | MyBioSource MBS224798 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RAT ANTI MOUSE CD16

catalog :

MBS224798

quantity :

0.25 mg

price :

430 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

[AT154-2]

reactivity :

mouse

application :

flow cytometry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS224798

products type :

Antibody

products full name :

RAT ANTI MOUSE CD16

products short name :

[CD16]

products name syn :

[FcRIII]

other names :

[Low affinity immunoglobulin gamma Fc region receptor III; Low affinity immunoglobulin gamma Fc region receptor III; low affinity immunoglobulin gamma Fc region receptor III; Fc receptor, IgG, low affinity III; Fc-gamma RIII; FcRIII; CD_antigen: CD16]

products gene name :

[CD16]

other gene names :

[Fcgr3; Fcgr3; CD16; IgG Fc receptor III; FcRIII]

uniprot entry name :

FCGR3_MOUSE

clonality :

Monoclonal

isotype :

IgG2b

clone :

[AT154-2]

host :

Rat

specificity :

Rat anti Mouse CD16 anitbody, clone AT154-2, recognizes CD16, also known as FcRIII. CD16 is a low affiniity activatory receptor which interacts preferentially with mouse IgG1, IgG2a and IgG2b immune complexes and is involved in various effector functions including phagocytosis, NK antibody dependent cellular cytotoxicity and the release of inflammatory mediators. In mice, CD16 is expressed on macrophages, NK cells, granulocytes, myeloid precursors and subpopulations of T lymphocytes.

form :

Purified IgG - liquid, TRIS buffered saline

storage stability :

Shelf Life: 18 months form date of dispatch. Store at 4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

tested application :

Flow Cytometry (FC), Immunohistology - Frozen

app notes :

Flow Cytometry: 1/10*. Immunohistology - Frozen: 1/100 - 1/1000. *Use 10ul of the suggested working dilution to label 1x10 6 cells in 100ul. Where this product has not been tested in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

other info1 :

Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. Preservative Stabilisers: 0.09 Sodium Azide (NaN 3 ). Concentration: IgG concentration 1.0 mg/ml. Target Species: Mouse. Immunogen: HEK293F cell expressing CD16 and gamma chain. Fusion Partners: Spleen cells from immunised rats were fused with cells of the NS-1 myeloma cell line

ncbi gi num :

119877

ncbi acc num :

P08508.1

uniprot acc num :

P08508

ncbi pathways :

Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway (1323740); Immune System Pathway (1323639); Innate Immune System Pathway (1323671); Leishmaniasis Pathway (144183); Leishmaniasis Pathway (144028); Osteoclast Differentiation Pathway (193149); Osteoclast Differentiation Pathway (193096); Phagosome Pathway (153914); Phagosome Pathway (153859); Regulation Of Actin Dynamics For Phagocytic Cup Formation Pathway (1323741)

uniprot summary :

FCGR2A: low affinity receptor for the Fc region of IgGs. Binding to IgG initiates cellular responses against pathogens and soluble antigens. A type I membrane protein found on monocytes, neutrophils and platelets. SHP-1 associates with this protein to modulate signaling events in myeloid cells. Protein type: Membrane protein, integral; Cell surface; Receptor, misc. Cellular Component: external side of plasma membrane; integral to membrane; membrane; plasma membrane. Molecular Function: IgG binding; IgG receptor activity. Biological Process: antibody-dependent cellular cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I; cell surface receptor linked signal transduction; mast cell activation; neutrophil chemotaxis; phagocytosis, engulfment; phagocytosis, recognition; positive regulation of phagocytosis; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of type I hypersensitivity; positive regulation of type IIa hypersensitivity; positive regulation of type III hypersensitivity; regulation of immune response; serotonin secretion

size1 :

0.25 mg

price1 :

430 USD

more info or order :

MyBioSource product webpage