antibody

MOUSE ANTI PIG CD3: Low Endotoxin

MBS224784

0.5 mg

675 USD

monoclonal

mouse

nonconjugated

[PPT3]

western blot, flow cytometry

Antibody

MOUSE ANTI PIG CD3: Low Endotoxin

[CD3]

[T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; CD_antigen: CD3e]

[CD3]

[CD3E; CD3E; CD3]

CD3E_PIG

Monoclonal

IgG1

[PPT3]

Mouse

Does not react with: Bovine, Goat, Horse, Human, Sheep

196

CD3 EPSILON

Low Endotoxin. Purified IgG - liquid

IgG concentration 1.0 mg/ml

Store at -20°C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch

Flow Cytometry, Immunohistology-Frozen, Immunoprecipitaion, Western Blotting, Functional Assays.

Flow Cytometry: 1/50-1/200. Use 10 uL of the suggested working dilution to label 1x10 6 cells in 100uL

Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. Buffer Solution: Phosphate buffered saline. Target Species: Pig. Preservative Stabiizers: None present. Endotoxin Level: <0.01 EU/ug. Immunogen: Porcine PBMCs. Carrier Free: yes

Mouse anti Pig CD3, clone PPT3 recognizes the porcine homologue of human CD3epsilon, a 24 kDa single pass type I membrane protein expressed by T-lymphocytes. Clone PPT3, also known under the clone designation FY1H2, was clustered at the second international swine CD workshop and found to specifically recognise an epitope on the porcine CD3epsilon designated as CD3c (Pescovitz, M.D., et al. 1998).CD3 is a multimeric protein complex composed of four distinct polypeptide chains (epsilon, gamma, delta, zeta) that assemble and function as three pairs of dimers (epsilongamma, epsilondelta, zetazeta). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Guy, C.S & Vignali, D.G. 2009). CD3 is a defining feature of cells belonging to the T cell lineage, antibodies recognising pig CD3 therefore provide useful markers of porcine T cells. Clone PPT3 has been demonstrated to recognise an epitope that is expressed both intracellularly and extracellularly, additionally clone PPT3 has been demonstrated to activate alpha/beta T-cells (Kirkham P.A., et al. 1996). Epitope mapping studies have demonstrated that clone PPT3 recognises an alternative epitope to clone PPT6 (Yang, H. et al. 1996; Pescovitz, M.D., et al. 1998). Clone PPT3 was tested on PBL from a range of other mammalian species and found to be negative suggesting that the epitope recognised by this clone is specific to porcine (Yang, H. et al. 1996).

78099944

Q7YRN2.1

Q7YRN2

Adaptive Immune System Pathway (1336882); Chagas Disease (American Trypanosomiasis) Pathway (147807); Chagas Disease (American Trypanosomiasis) Pathway (147795); Costimulation By The CD28 Family Pathway (1336888); Downstream TCR Signaling Pathway (1336887); Generation Of Second Messenger Molecules Pathway (1336886); HTLV-I Infection Pathway (373897); HTLV-I Infection Pathway (373889); Hematopoietic Cell Lineage Pathway (84490); Hematopoietic Cell Lineage Pathway (489)

The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses.

0.5 mg

675 USD