RAT ANTI MOUSE CD64 | MyBioSource MBS224780 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RAT ANTI MOUSE CD64

catalog :

MBS224780

quantity :

0.25 mg

price :

430 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

[AT152-9]

reactivity :

mouse

application :

immunohistochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS224780

products type :

Antibody

products full name :

RAT ANTI MOUSE CD64

products short name :

[CD64]

products name syn :

[FcRI, Fcg1]

other names :

[High affinity immunoglobulin gamma Fc receptor I; High affinity immunoglobulin gamma Fc receptor I; high affinity immunoglobulin gamma Fc receptor I; Fc receptor, IgG, high affinity I; Fc-gamma RI; FcRI; CD_antigen: CD64]

products gene name :

[CD64]

other gene names :

[Fcgr1; Fcgr1; CD64; IGGHAFC; AI323638; AV092959; FcgammaRI; Fcg1; IgG Fc receptor I; FcRI]

uniprot entry name :

FCGR1_MOUSE

clonality :

Monoclonal

isotype :

IgG2a

clone :

[AT152-9]

host :

Rat

reactivity :

Mouse

sequence length :

404

specificity :

CD64

form :

Purified IgG-liquid

concentration :

IgG concentration 1.0 mg/ml

storage stability :

Store at +4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch

tested application :

Immunohistochemistry (IHC) Formalin, Flow Cytometry (FC/FACS)

app notes :

Immunohistochemistry- Frozen: 1/1000. Flow Cytometry: 1/10. Flow Cytometry Note: Use 10ul of the suggested working dilution to label 1 x 10 6 cells in 100ul

other info1 :

Preparation: Purified IgG prepared by ion exchange chromatography from tissue culture supernatant. Preservative Stabilisers: 0.09% Sodium Azide (NaN 3 ) . Buffer Solution: TRIS buffered saline

other info2 :

Target Species: Mouse. Immunogen: HEK293F cells expressing CD64 and gamma chain. Fusion Partners: Spleen cells from immunised rats were fused with cells of the NS-1 myeloma cell line.

products description :

Rat anti mouse CD64 antibody, clone AT152-9, recognizes mouse CD64 also known as FcRI. CD64 is a high affinity activatory receptor for IgG2a and a low affinity receptor for IgG2b and IgG3 type antibodies. The interaction between Fc receptors and antibodies play important roles in both the innate and adaptive immune responses. CD64, through binding of the Fc segment of IgG, mediates phagocytosis and plays a role in antibody-dependent cellular cytotoxicity and clearance of immune complexes. In addition, CD64 also functions as an antigen capture for presentation to T-cells and also mediates the release of cytokines and reactive oxygen intermediates including interleukin (IL)-1, IL-6 and tumor necrosis factor (TNF) alpha. It is constitutively expressed on monocytes and macrophages, germinal centre dendritic cells and early myeloid lineage cells, but not lymphocytes. Expression on monocytes can be strongly upregulated by treatment with interferon (IFN) gamma or G-CSF, and can be induced on neutrophils and eosinophils by IFN gamma.

ncbi gi num :

119873

ncbi acc num :

P26151.1

uniprot acc num :

P26151

ncbi pathways :

Adaptive Immune System Pathway (1323640); Antigen Processing-Cross Presentation Pathway (1323665); Class I MHC Mediated Antigen Processing Presentation Pathway (1323662); Cross-presentation Of Soluble Exogenous Antigens (endosomes) Pathway (1323667); Fc Gamma R-mediated Phagocytosis Pathway (114297); Fc Gamma R-mediated Phagocytosis Pathway (106590); Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway (1323740); Hematopoietic Cell Lineage Pathway (83275); Hematopoietic Cell Lineage Pathway (489); Immune System Pathway (1323639)

uniprot summary :

FCGR1A: High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Belongs to the immunoglobulin superfamily. FCGR1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, misc. Cellular Component: external side of plasma membrane; integral to membrane; lipid raft; membrane; plasma membrane. Molecular Function: IgG binding; IgG receptor activity; leukotriene receptor binding. Biological Process: antibody-dependent cellular cytotoxicity; antigen processing and presentation of exogenous antigen; antigen processing and presentation of exogenous peptide antigen via MHC class I; cell surface receptor linked signal transduction; defense response to bacterium; immune system process; innate immune response; phagocytosis, engulfment; phagocytosis, recognition; positive regulation of phagocytosis; positive regulation of phagocytosis, engulfment; positive regulation of type IIa hypersensitivity; positive regulation of type III hypersensitivity; receptor-mediated endocytosis; regulation of immune response; response to bacterium

size1 :

0.25 mg

price1 :

430 USD

more info or order :

MyBioSource product webpage