antibody

MOUSE ANTI HUMAN CD68

MBS224643

0.2 mg

415 USD

monoclonal

mouse

nonconjugated

KP1

human, rat

western blot, immunocytochemistry, immunoprecipitation, flow cytometry, FACS

Antibody

MOUSE ANTI HUMAN CD68

CD68

macrosialin isoform B; Macrosialin; macrosialin; CD68 antigen; macrophage antigen CD68; scavenger receptor class D, member 1; CD68 molecule; Gp110; CD_antigen: CD68

CD68

CD68; CD68; GP110; LAMP4; SCARD1

CD68_HUMAN

Monoclonal

IgG1

KP1

Mouse

Primate, Rat

327

Purified. Purified IgG - liquid

IgG concentration 1.0mg/ml

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR. . Immunohistology - Paraffin: Application Note: This antibody requires heat-mediated antigen retrieval prior to staining paraffin sections. Citrate buffer, pH6.0, is recommended for this purpose .

Perservative Stabilisers: 0.09% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution: Phosphate buffered saline. Target Species: Human

Mouse anti human CD68 antibody, clone KP1 recognises the human CD68 cell surface antigen, a 110kD glycoprotein primarily expressed by macrophages and monocytes.

91199550

NP_001035148.1

NM_001040059.1

P34810

34,654 Da

Lysosome Pathway (99052); Lysosome Pathway (96865)

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell surface; Membrane protein, integral. Chromosomal Location of Human Ortholog: 17p13. Cellular Component: membrane; lysosomal membrane; integral to membrane; plasma membrane; endosome membrane

0.2 mg

415 USD