antibody

MOUSE ANTI PIG CD163

MBS224621

0.1 mg

355 USD

monoclonal

mouse

nonconjugated

[2A10/11]

western blot, immunocytochemistry, immunoprecipitation, flow cytometry, FACS

Antibody

MOUSE ANTI PIG CD163

[CD163]

[scavenger receptor cysteine-rich type 1 protein M130; Scavenger receptor cysteine-rich type 1 protein M130; scavenger receptor cysteine-rich type 1 protein M130; CD_antigen: CD163Soluble CD163; sCD163]

[CD163]

[CD163; CD163; M130; sCD163]

C163A_PIG

Monoclonal

IgG1

[2A10/11]

Mouse

1110

Purified IgG - liquid. Phosphate buffered saline

IgG concentration 1.0 mg/ml

Store at 4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen, Immunohistology-Paraffin , Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)

Flow Cytometry: Use 10ul of the suggested working dilution to 1x10 6 cells in 100ul. Western Blot: Clone 2A10/11 detects a band of approximately 120kD in alveolar macrophage cell lysates under non-reducing conditions. Flow Cytometry: 1/50-1/200

Testing Data #1

Testing Data #2

Perservative Stabilisers: 0.09% Sodium Azide (NaN3). Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag.8.653 myeloma cell line.

Target Species: Pig. Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. Carrier free: Yes. Immunogen: Porcine alveolar macrophages.

Mouse anti Pig CD163 antibody, clone 2A10/11 recognises porcine CD163, a 120kD single pass type 1 transmembrane cell surface glycoprotein expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative. Expression of CD163 on peripheral blood monocytes varies between about 5% and 50% depending on the donor (Sanchez et al. 1999). Mouse anti Pig CD163, clone 2A10/11 is reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner (Sanchez-Torres et al. 2003).

47522778

NP_999141.1

NM_213976.1

Q2VL90

Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1 (). May play a role in the process of infection of porcine monocytes/macrophages by African swine fever virus (ASFV). In case of porcine reproductive and respiratory syndrome virus (PRRSV), serves mediates virion attachment and plays a role in viral entry.

0.1 mg

355 USD