MOUSE ANTI HUMAN GLUTAMATE DECARBOXYLASE 2 (N-TERMINAL) | MyBioSource MBS224587 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN GLUTAMATE DECARBOXYLASE 2 (N-TERMINAL)

catalog :

MBS224587

quantity :

0.1 mg

price :

280 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

N-GAD65

reactivity :

human

application :

western blot, immunoprecipitation, radioimmunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS224587

products type :

Antibody

products full name :

MOUSE ANTI HUMAN GLUTAMATE DECARBOXYLASE 2 (N-TERMINAL)

products short name :

GLUTAMATE DECARBOXYLASE 2

other names :

glutamate decarboxylase 2; Glutamate decarboxylase 2; glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform

other gene names :

GAD2; GAD2; GAD65; GAD65; GAD-65

uniprot entry name :

DCE2_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

N-GAD65

host :

Mouse

sequence length :

585

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1.0mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunoprecipitation (IP), Immunohistology Paraffin, Radioimmunoassays (RIA), Western Blot (WB)

app notes :

Western Blotting: Minimum Dilution: 1/100; Maximum Dilution: 1/1000

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

other info2 :

Histology Positive Control Tissue: Human brain. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

Mouse anti Human Glutamate Decarboxylase 2 (N-Terminal) antibody, clone N-GAD65 recognizes human glutamate decarboxylase 2, also known as 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform or GAD-65. Glutamate decarboxylase 2 is a 585 amino acid ~65 kDa lipid anchored membrane associated protein responsible for catalysis of gamma -aminobutyric acid (GABA) production, the major inhibitory neurotransmitter in the central nervous system (Fenalti et al. 2007) and is expressed in the brain and pancreatic beta cells. Glutamate decarboxylase 2 is the 65 kDa isoform of glutamate decarboxylase, encoded by the GAD2 gene and predominantly expressed by nerve termini, as opposed to the 67 kDa isoform (GAD67), predominantly found in the cell body, and encoded by the GAD1 gene. Autoantibodies to Glutamate decarboxylase 2 are detected in autoimmune diseases, including Graves disease (Kallmann et al. 1997), Stiff Man Syndrome (Raju et al. 2005) and are most prevalent in patients with Type I diabetes mellitus or those at high risk of developing Type I diabetes (Petersen et al. 1994). The N-Terminal region of GAD65 is essential for targeting the enzyme to GABA-containing secretory vesicles (Shi et al. 1994). Mouse anti Human Glutamate Decarboxylase 2 (N-Terminal) antibody, clone N-GAD65 recognizes GAD65 but does not recognize GAD67 (Hampe et al. 2001).

ncbi gi num :

4503875

ncbi acc num :

NP_000809.1

ncbi gb acc num :

NM_000818.2

uniprot acc num :

Q05329

ncbi mol weight :

65,411 Da

ncbi pathways :

Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Biogenic Amine Synthesis Pathway (198793); Butanoate Metabolism Pathway (83007); Butanoate Metabolism Pathway (391); GABA (gamma-Aminobutyrate) Shunt Pathway (413433); GABA (gamma-Aminobutyrate) Shunt Pathway (468220); GABA Synthesis Pathway (187175); GABA Synthesis, Release, Reuptake And Degradation Pathway (187174)

ncbi summary :

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

uniprot summary :

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family. Protein type: Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - alanine, aspartate and glutamate; Lyase; Carbohydrate Metabolism - butanoate; EC 4.1.1.15; Other Amino Acids Metabolism - taurine and hypotaurine. Chromosomal Location of Human Ortholog: 10p11.23. Cellular Component: presynaptic membrane; Golgi membrane; synaptic vesicle membrane; perinuclear region of cytoplasm; axon; plasma membrane; cell junction; cytosol. Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding. Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process

size1 :

0.1 mg

price1 :

280 USD

more info or order :

MyBioSource product webpage