MOUSE ANTI HUMAN CD11c:FITC | MyBioSource MBS224506 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN CD11c:FITC

catalog :

MBS224506

quantity :

0.025 mg

price :

175 USD

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

[BU15]

reactivity :

human

application :

flow cytometry, FACS

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS224506

products type :

Antibody

products full name :

MOUSE ANTI HUMAN CD11c:FITC

products short name :

[CD11c]

other names :

[integrin alpha-X isoform 2; Integrin alpha-X; integrin alpha-X; CD11 antigen-like family member C; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); leu M5, alpha subunit; leukocyte adhesion glycoprotein p150,95 alpha chain; leukocyte adhesion receptor p150,95; leukocyte surface antigen p150,95, alpha subunit; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain; integrin, alpha X (complement component 3 receptor 4 subunit); CD11 antigen-like family member C; Leu M5; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; CD_antigen: CD11c]

products gene name :

[CD11c]

other gene names :

[ITGAX; ITGAX; CD11C; SLEB6; CD11C]

uniprot entry name :

ITAX_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[BU15]

host :

Mouse

reactivity :

Cynomolgus monkey; N.B. Antibody reactivity and working conditions may vary between species.

sequence length :

1163

specificity :

Mouse anti Human CD11c antibody, clone Bu15 recognizes the integrin alphax subunit (ITGAX), a ~150 kDa glycoprotein also known as CD11 antigen-like family member C or Leu M5, CD11c is expressed by macrophages, monocytes, NK cells and most dendritic cells (Kohrgruber et al. 1999). CD11c is also expressed at a lower level by granulocytes. CD11c forms a heterodimeric integral membrane protein with the integrin beta 2 chain to form the leukocyte specific integrin 'inactivated-C3b receptor 4'. CD11c interacts with a number of ligands including the G-P-R sequence in fibrinogen, ICAM-1 (Frick et al. 2005), iC3b (Sadhu et al. 2007) and Junctional adhesion molecule-like (Bazzoni et al. 2011). CD11c is involved in cell-cell interactions during the inflammatory process and is important for monocyte adhesion and chemotaxis. CD11c also acts as a signalling receptor for polysaccharide (Inqualls et al. 1995). CD11c is expressed by hairy cell leukaemia cells (Goodman et al. 2003; Nicolaou et al. 2003).

form :

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

concentration :

IgG concentration 0.1 mg/ml

storage stability :

Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

tested application :

Flow cytometry (FC/FACS)

app notes :

Flow Cytometry: Min. Dilution: Neat-Max. Dilution: 1/10; Use 10ul of the suggested working dilution to label 10 6 cells in 100ul. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. 1% Bovine Serum Albumin. Preparation: Purified IgG prepared from tissue culture supernatant

other info2 :

Buffer Solution: Phosphate buffered saline. Target Species: Human. Max Ex/Em: Fluorophore: FITC. Excitation Max (nm): 490. Emission Max (nm): 525

ncbi gi num :

34452173

ncbi acc num :

NP_000878.2

ncbi gb acc num :

NM_000887.4

uniprot acc num :

P20702

ncbi pathways :

Cell Surface Interactions At The Vascular Wall Pathway (106062); ECM Proteoglycans Pathway (833812); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795); Hemostasis Pathway (106028); Integrin Cell Surface Interactions Pathway (106110); Integrin-mediated Cell Adhesion Pathway (198816); Regulation Of Actin Cytoskeleton Pathway (83089); Regulation Of Actin Cytoskeleton Pathway (500); Tuberculosis Pathway (213780)

ncbi summary :

This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

uniprot summary :

ITGAX: Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. Belongs to the integrin alpha chain family. Protein type: Motility/polarity/chemotaxis; Membrane protein, integral. Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: cell surface; membrane; plasma membrane; integrin complex. Molecular Function: protein binding; metal ion binding; receptor activity. Biological Process: integrin-mediated signaling pathway; heterotypic cell-cell adhesion; extracellular matrix organization and biogenesis; organ morphogenesis; blood coagulation; cell adhesion; leukocyte migration. Disease: Systemic Lupus Erythematosus

size1 :

0.025 mg

price1 :

175 USD

more info or order :

MyBioSource product webpage