antibody

MOUSE ANTI PIG SLA CLASS II DR

MBS224479

0.1 mg

350 USD

monoclonal

mouse

nonconjugated

2E9/13

immunoprecipitation, flow cytometry, FACS

Antibody

MOUSE ANTI PIG SLA CLASS II DR

SLA CLASS II DR

Monoclonal

IgG2b

2E9/13

Mouse

Reacts with: Bovine. N.B. Antibody reactivity and working conditions may vary between species.

Purified. Purified IgG - liquid

IgG concentration 1.0 mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)

Flow Cytometry: Use 10ul of the suggested working dilution to 1x106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200

Perservative Stabilisers: 0.09% Sodium Azide (NaN3). Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. Immunogen: Porcine monocytes. Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse myeloma cell line.

Buffer Solution: Phosphate buffered saline. Target Species: Pig

Mouse anti Pig SLA Class II DR antibody, clone 289/13 recognizes SLA DR molecules which are expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells. Mouse anti Pig SLA Class II DR antibody, clone 289/13 reacts with lymphocytes from all outbred and miniature pigs so far tested, suggesting that it recognizes a monomorphic determinant of porcine SLA DR. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. There are 3 major MHC class II proteins encoded by the SLA which are SLA DP, SLA DQ and SLA DR. Mouse anti pig SLA class II Dr, clone 2E9/13 immunoprecipitates a heterodimer composed of two polypeptides of 28 and 35kD from NP-40 extracts of biotin surface-labelled porcine peripheral blood mononuclear cells. Clone 2E9/13 is reported to inhibit the mixed lymphocyte reaction and T cell stimulation induced by African swine fever virus and staphylococcal enterotoxin B (Bullido et al. 1997).

0.1 mg

350 USD