catalog number :
MBS224009
products full name :
GUINEA PIG ANTI PIG INSULIN
products short name :
INSULIN
other names :
insulin; Insulin; insulin; preproinsulin
other gene names :
INS; INS
uniprot entry name :
INS_PIG
form :
Purified. Purified IgG - liquid
concentration :
IgG concentration 5.0mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Immunohistology Frozen, ELISA (EIA), Immunohistology Paraffin
app notes :
Immunohistology - Frozen: Minimum Dilution: 1/50; Maximum Dilution: 1/1600
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
other info2 :
Histology Positive Control Tissue: Pancreas. Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to pig insulin were raised by repeated immunisations of Guinea Pigs with highly purified antigen. Ig fraction prepared from whole sruem by ammonium sulphate precipitation. Target Species: Pig
products description :
Guinea pig anti Pig insulin antibody recognizes porcine insulin, a hormone secreted by the beta cells of the islets of Langerhans in the pancreas. Insulin belongs to a family of hormones including the insulin-like growth hormones and the relaxins. The insulin precursor is a 108amino acid polypeptide consisting of a 24 aa signal peptide, an insulin A chain, a prohormone polypeptide (also known as the ˜C chain') and an insulin B chain together having a predicted molecular mass of 11,672 Da. Active insulin migrates with an apparent molecular mass of 6kDa in western blotting of porcine, bovine and lapine samples (Schechter et al. 1992). Insulin is a hormone that functions in carbohydrate homeostasis, and acts to decrease blood glucose concentration by increasing cell permeability to monosaccharides, amino acids and fatty acids, and accelerate glycolysis and glycogen synthesis in the liver, it induces uptake of glucose into cells through its interaction with the glucose transporter GLUT4 (Watson and Pressin 2001). Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumours of B-cell origin such as insulinoma (Schiel et al. 2006). Guinea pig anti pig insulin antibody has been successfully used to demonstrate the presence og insulin in the islets of Langerhans of murine pancreas using immunohistochemistry on paraffin embedded material (Orts ter et al. 2012).
ncbi acc num :
NP_001103242.1
ncbi gb acc num :
NM_001109772.1
ncbi mol weight :
11,672 Da
ncbi pathways :
AMPK Signaling Pathway 989938!!AMPK Signaling Pathway 992181!!Aldosterone-regulated Sodium Reabsorption Pathway 130624!!Aldosterone-regulated Sodium Reabsorption Pathway 130590!!FoxO Signaling Pathway 921549!!HIF-1 Signaling Pathway 695240!!Insulin Secretion Pathway 777548!!Insulin Signaling Pathway 84501!!Insulin Signaling Pathway 501!!Maturity Onset Diabetes Of The Young Pathway 84507