GOAT ANTI HUMAN CD274 (C-TERMINAL) | MyBioSource MBS223738 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

GOAT ANTI HUMAN CD274 (C-TERMINAL)

catalog :

MBS223738

quantity :

0.1 mg

price :

500 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS223738

products type :

Antibody

products full name :

GOAT ANTI HUMAN CD274 (C-TERMINAL)

products short name :

CD274

other names :

programmed cell death 1 ligand 1 isoform b; Programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; CD274 molecule; B7 homolog 1; B7-H1; CD_antigen: CD274

products gene name :

CD274

other gene names :

CD274; CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1; B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1

uniprot entry name :

PD1L1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Goat

sequence length :

176

specificity :

This item specifically recognises an epitope within the C-terminal (CT) region of human CD274, otherwise known as PD-L1, a cell surface glycoprotein and member of the B7 family of co-stimulatory molecules, constitutively expressed on macrophages and dendritic cells, and inducible on activated T-cells, B-cells, endothelial cells and epithelial cells, in response to interferons. CD274 acts as both a co-stimulatory and co-inhibitory molecule, co-stimulating early T cell priming, differentiation and IL-10 production, and inhibiting T cell proliferation and TCR-mediated activation of IL-2 production, through interaction with the receptor CD279/PD-1. Studies have linked the deregulation of CD274 function with many autoimmune diseases and cancers, as well as chronic viral and intracellular bacterial infection.

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 0.5mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)

app notes :

Western Blot: This item detects a band of approximately 35kDa in human heart cell lysates. ELISA: Minimum Dilution: 1/8000. Immunohistology - Paraffin: Minimum Dilution: 4.0; Maximum Dilution: 6.0ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. . Western Blotting: Minimum Dilution: 0.3; Maximum Dilution: 1.0ug/ml

other info1 :

Perservative Stabilisers: 0.02% Sodium Azide (NaN3). 0.5% Bovine Serum Albumin

other info2 :

Immunogen: Synthetic peptide sequence CKKQSDTHLEET from the C-Terminal region of CD274 (NP_054862.1). Histology Positive Control Tissue: Human skeletal muscle. Buffer Solution: TRIS buffered saline. Antiserum Preparation: Antiserum to human CD274 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography. Target Species: Human

products description :

MBS223738 specifically recognises an epitope within the C-terminal (CT) region of human CD274, otherwise known as PD-L1, a cell surface glycoprotein and member of the B7 family of co-stimulatory molecules, constitutively expressed on macrophages and dendritic cells, and inducible on activated T-cells, B-cells, endothelial cells and epithelial cells, in response to interferons. CD274 acts as both a co-stimulatory and co-inhibitory molecule, co-stimulating early T cell priming, differentiation and IL-10 production, and inhibiting T cell proliferation and TCR-mediated activation of IL-2 production, through interaction with the receptor CD279/PD-1. Studies have linked the deregulation of CD274 function with many autoimmune diseases and cancers, as well as chronic viral and intracellular bacterial infection.

ncbi gi num :

390979639

ncbi acc num :

NP_001254635.1

ncbi gb acc num :

NM_001267706.1

uniprot acc num :

Q9NZQ7

ncbi mol weight :

20,454 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Costimulation By The CD28 Family Pathway (119552); Immune System Pathway (106386); PD-1 Signaling Pathway (119557)

uniprot summary :

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 9p24. Cellular Component: plasma membrane; integral to membrane; endomembrane system; external side of plasma membrane. Molecular Function: protein binding. Biological Process: regulation of activated T cell proliferation; cell surface receptor linked signal transduction; negative regulation of interleukin-10 production; negative regulation of interferon-gamma production; negative regulation of activated T cell proliferation; T cell costimulation; immune response; positive regulation of T cell proliferation; signal transduction

size1 :

0.1 mg

price1 :

500 USD

more info or order :

MyBioSource product webpage