antibody

SHEEP ANTI 5-METHYLCYTOSINE:Preservative Free

MBS223530

0.1 mg

325 USD

polyclonal

sheep

nonconjugated

western blot, immunocytochemistry, immunoprecipitation

Antibody

SHEEP ANTI 5-METHYLCYTOSINE:Preservative Free

5-METHYLCYTOSINE

Polyclonal

IgG

Sheep

Preservative Free. Purified IgG - liquid

IgG concentration 1.0mg/ml

Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen*, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)

Immunohistology - Frozen: Minimum Dilution: 10ug/ml; Application Note: Using a fixation step in cold methanol for 30 minutes followed by immersion in 0.007 M NaOH for 10-15 seconds allows staining with the simultaneous detection of nuclear cytoplasmic and membrane components, as well as preservation of morphological detail. . Immunoprecipitation: Minimum Dilution: 25; Maximum Dilution: 100ug/ml;. Immunohistology - Paraffin: Minimum Dilution: 10ug/ml; Application Note: * Using a fixation step in cold methanol for 30 minutes followed by immersion in 0.007 M NaOH for 10-15 seconds allows staining with the simultaneous detection of nuclear cytoplasmic and membrane components, as well as preservation of morphological detail. . Western Blotting: Minimum Dilution: 1:1000

Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antiserum to 5-MeC was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography. Target Species: Broad

Sheep Anti 5-Methylcytosine (5-MeC) recognizes the methylated form of cytosine (C), an epigenetic modification catalyzed by DNA methyltransferases resulting in the attachment of a methyl group to carbon 5. This modification alters the structure of cytosine but not its base-pairing properties. The timing and function of cytosine methylation varies amongst species with roles in such processing events as transcription and recombination. For example, in mammals, methylation occuring during early development and emrbyogensis functions in X chromosome inactivation and gene imprinting, wherease in plants, DNA methylation occurs later and affects genomic stability through silencing of transposable elements (He et al. 2011).The deamination of 5-MeC results in the formation of thymine (T) instead of uracil. Since thymine is not recognized by DNA repair enzymes, cytosine to thymine conversions can cause transition mutations. The most frequent mutations in human genetic diseases are those involving C to T transitions occurring at CG sites (Morgan et al. 2004). Another type of mutation involving 5-MeC involves the formation of pyrimidine dimers. Upon exposure to sunlight, pyrimidine dimers form preferentially at dipyrimidine sequences containing 5-MeC, a mutation frequently seen in human skin cancers (Pfeifer et al. 2005). Sheep Anti-5-MeC is reported as suitable for use in immunocytochemistry.

0.1 mg

325 USD