antibody

RABBIT ANTI HUMAN I-TAC

MBS223529

0.1 mg

540 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, enzyme immunoassay

Antibody

RABBIT ANTI HUMAN I-TAC

I-TAC

C-X-C motif chemokine 11 isoform 2; C-X-C motif chemokine 11; C-X-C motif chemokine 11; beta-R1; interferon gamma-inducible protein 9; interferon-inducible T-cell alpha chemoattractant; small inducible cytokine B11; small inducible cytokine subfamily B (Cys-X-Cys), member 11; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; small-inducible cytokine B11; chemokine (C-X-C motif) ligand 11; Beta-R1; H174; Interferon gamma-inducible protein 9; IP-9; Interferon-inducible T-cell alpha chemoattractant; I-TAC; Small-inducible cytokine B11

I-TAC

CXCL11; CXCL11; IP9; H174; IP-9; b-R1; I-TAC; SCYB11; SCYB9B; ITAC; SCYB11; SCYB9B; IP-9; I-TAC

CXL11_HUMAN

Polyclonal

IgG

Rabbit

106

Purified. Purified IgG - lyophilised

IgG concentration 1.0mg/ml after reconstitution

Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of reconstitution.

ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin*, Western Blot (WB)

ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml;. Functional Assays: Minimum Dilution: 7.0; Maximum Dilution: 8.0ug/ml;. Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. . Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml

Reconstitution: Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

Immunogen: Recombinant human I-TAC. Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antiserum to human I-TAC were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. Target Species: Human

Rabbit anti Human I-TAC antibody specifically recognizes human I-TAC (Interferon-inducible T-cell alpha chemoattractant), otherwise known as CXCL11, a member of the CXC chemokine family, which signals through binding to CD183 (CXCR3), and is regulated by interferon gamma and beta. I-TAC is highly expressed by peripheral blood leucocytes, the pancreas and liver, and acts as a potent chemoattractant for IL-2 activated T cells, as well as a natural antagonist for CD195 (CCR5).

693074655

NP_001289052.1

NM_001302123.1

O14625

10,365 Da

CXCR3-mediated Signaling Events Pathway (138011); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (i) Signalling Events Pathway (119550)

Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC. This antimicrobial gene is a CXC member of the chemokine superfamily. Its encoded protein induces a chemotactic response in activated T-cells and is the dominant ligand for CXC receptor-3. The gene encoding this protein contains 4 exons and at least three polyadenylation signals which might reflect cell-specific regulation of expression. IFN-gamma is a potent inducer of transcription of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

CXCL11: Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses. Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q21.2. Cellular Component: extracellular space; extracellular region. Molecular Function: heparin binding; protein binding; CXCR3 chemokine receptor binding; chemokine activity. Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; positive regulation of leukocyte chemotaxis; immune response; response to lipopolysaccharide; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of cAMP metabolic process; inflammatory response; signal transduction; chemotaxis; regulation of cell proliferation

0.1 mg

540 USD