antibody

RABBIT ANTI DAI

MBS223498

0.1 mg

365 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, enzyme immunoassay

Antibody

RABBIT ANTI DAI

DAI

Z-DNA-binding protein 1 isoform b; Z-DNA-binding protein 1; Z-DNA-binding protein 1; DNA-dependent activator of IFN-regulatory factors; DNA-dependent activator of IRFs; DNA-dependent activator of interferon regulatory factors; tumor stroma and activated macrophage protein DLM-1; Z-DNA binding protein 1; Tumor stroma and activated macrophage protein DLM-1

DAI

ZBP1; ZBP1; DAI; DLM1; DLM-1; C20orf183; C20orf183; DLM1

ZBP1_HUMAN

Polyclonal

IgG

Rabbit

Mouse, Rat

428

This product recognizes human DAI, also known as Z-DNA Binding Protein (ZBP1), a 46kDa DNA binding protein belonging to a family of proteins that contain a Za domain which specifically binds to the left-handed Z-DNA and Z-RNA. Initial studies suggested that DAI may be involved in processes such host response to neoplasia. Additional research has indicated that the cytosolic DAI can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of DAI to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK). DAI is highly expressed in lymphatic tissues such as lymph nodes, leukocytes, tonsil, bone marrow and spleen. Expression at a lower level is also seen in thymus, lung and liver. It has been demonstrated that multiple isoforms of DAI exist.

Purified. Purified IgG - liquid

IgG concentration 1.0mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)*

Western Blot: This item detects a band of approximately 46 kDa in mouse small intestine tissue lysate. Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. . Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml; Application Note: Reduced and denatured conditions

Perservative Stabilisers: 0.02% Sodium Azide (NaN3)

Immunogen: Synthetic peptide corresponding to an 18 amino acid sequence from near the carboxy terminus of human ZBP1. Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to human DAI were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography. Target Species: Human

This product recognizes human DAI, also known as Z-DNA Binding Protein (ZBP1), a 46kDa DNA binding protein belonging to a family of proteins that contain a Za domain which specifically binds to the left-handed Z-DNA and Z-RNA. Initial studies suggested that DAI may be involved in processes such host response to neoplasia. Additional research has indicated that the cytosolic DAI can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of DAI to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK). DAI is highly expressed in lymphatic tissues such as lymph nodes, leukocytes, tonsil, bone marrow and spleen. Expression at a lower level is also seen in thymus, lung and liver. It has been demonstrated that multiple isoforms of DAI exist.

238550208

NP_001153889.1

NM_001160417.1

Q9H171

38,220 Da

Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); IRF3 Mediated Activation Of Type 1 IFN Pathway (576257); IRF3-mediated Induction Of Type I IFN Pathway (833819); Immune System Pathway (106386); Innate Immune System Pathway (106387); RIP-mediated NFkB Activation Via ZBP1 Pathway (576258); Regulation Of Innate Immune Responses To Cytosolic DNA Pathway (833823); STING Mediated Induction Of Host Immune Responses Pathway (685548)

This gene encodes a Z-DNA binding protein. The encoded protein plays a role in the innate immune response by binding to foreign DNA and inducing type-I interferon production. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

ZBP1: Participates in the detection by the host s innate immune system of DNA from viral, bacterial or even host origin. Plays a role in host defense against tumors and pathogens. Acts as a cytoplasmic DNA sensor which, when activated, induces the recruitment of TBK1 and IRF3 to its C-terminal region and activates the downstream interferon regulatory factor (IRF) and NF-kappa B transcription factors, leading to type-I interferon production. ZBP1-induced NF-kappaB activation probably involves the recruitment of the RHIM containing kinases RIPK1 and RIPK3. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; DNA-binding. Chromosomal Location of Human Ortholog: 20q13.31. Cellular Component: cytoplasm; nucleus; cytosol. Molecular Function: double-stranded RNA adenosine deaminase activity; protein binding; DNA binding; left-handed Z-DNA binding; RNA binding. Biological Process: metabolic process; positive regulation of interferon type I production; innate immune response

0.1 mg

365 USD