antibody

RABBIT ANTI NMDA RECEPTOR/NR1 SUBUNIT/SPLICE VARIANT N1

MBS223269

0.025 mg

610 USD

polyclonal

rabbit

nonconjugated

human, mouse, dog, chicken

Antibody

RABBIT ANTI NMDA RECEPTOR/NR1 SUBUNIT/SPLICE VARIANT N1

NMDAR/NR1/N1

glutamate receptor ionotropic, NMDA 1 isoform 2; Glutamate receptor ionotropic, NMDA 1; glutamate receptor ionotropic, NMDA 1; N-methyl-D-aspartate receptor subunit NR1; glutamate [NMDA] receptor subunit zeta-1; glutamate receptor, ionotropic, NMDA1 (zeta 1); Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1

NMDA

Grin1; Grin1; NR1; GluN1; Nmdar; NMD-R1; NMDAR1; M100174; Rgsc174; GluRzeta1; GluRdelta1; Glurz1; GluN1; NMD-R1

NMDZ1_MOUSE

Polyclonal

IgG

Rabbit

Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Monkey (Expected from Sequence), Mouse, Xenopus (Expected from Sequence)

906

This item specifically recognises the 120kDa NR1 subunit of the NMDA (N-methyl-D-aspartate) receptor, containing the N1 splice variant insert. This antibody does not recognize the NR1 subunit, where the N1 splice variant insert is absent. Receptors for NMDA (NMDAR) belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+ permeability. The NR1 subunit has eight splice variants, arising from either the deletion or insertion of three exon cassettes within the C-terminal (C1, C2) and N-terminal (N1) NR1 domains. These splice variants differ in both their expression and pharmacological properties.

Purified. Purified IgG - lyophilised

Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of reconstitution.

Immunohistology Frozen, Western Blot (WB)

Immunohistology - Frozen: Minimum Dilution: 1/1000; Maximum Dilution: 1/2000;. Western Blotting: Maximum Dilution: 1/1000

Reconstitution: Reconstitute with 50ul phosphate buffered saline (PBS). Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.

Immunogen: Synthetic peptide from rat NMDA receptor NR1 subunit, containing the N1 splice variant insert. Antiserum Preparation: Antisera to rat NMDA receptor, NR1 subunit, splice variant N1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. Target Species: Rat

MBS223269 specifically recognises the 120kDa NR1 subunit of the NMDA (N-methyl-D-aspartate) receptor, containing the N1 splice variant insert. This antibody does not recognize the NR1 subunit, where the N1 splice variant insert is absent. Receptors for NMDA (NMDAR) belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+ permeability. The NR1 subunit has eight splice variants, arising from either the deletion or insertion of three exon cassettes within the C-terminal (C1, C2) and N-terminal (N1) NR1 domains. These splice variants differ in both their expression and pharmacological properties.

294997257

NP_001171127.1

NM_001177656.2

99,423 Da

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (1111226); Alcoholism Pathway (585577); Alcoholism Pathway (587116); Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Amphetamine Addiction Pathway (547634); Amphetamine Addiction Pathway (550546); Amyotrophic Lateral Sclerosis (ALS) Pathway (83296); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Axon Guidance Pathway (1111058)

NMDAR1: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. Three alternatively-spliced isoforms have been described. Protein type: Membrane protein, integral; Channel, ligand-gated; Channel, calcium; Membrane protein, multi-pass. Cellular Component: cell surface; cell; integral to plasma membrane; endoplasmic reticulum; dendrite; postsynaptic density; dendritic spine; integral to membrane; terminal button; excitatory synapse; N-methyl-D-aspartate selective glutamate receptor complex; postsynaptic membrane; synaptic vesicle; growth cone; membrane; cell soma; cytoplasm; plasma membrane; synapse; cell junction. Molecular Function: protein dimerization activity; voltage-gated cation channel activity; neurotransmitter binding; glutamate receptor binding; calcium channel activity; ion channel activity; receptor activity; calcium ion binding; calmodulin binding; protein binding; enzyme binding; extracellular-glutamate-gated ion channel activity; glutamate binding; ionotropic glutamate receptor activity; protein heterodimerization activity; N-methyl-D-aspartate selective glutamate receptor activity; glycine binding; cation channel activity; receptor binding. Biological Process: regulation of long-term neuronal synaptic plasticity; male mating behavior; startle response; prepulse inhibition; positive regulation of apoptosis; adult locomotory behavior; regulation of dendrite morphogenesis; rhythmic process; response to morphine; regulation of synaptic plasticity; regulation of axonogenesis; sensory perception of pain; calcium ion homeostasis; synaptic transmission; regulation of respiratory gaseous exchange; learning and/or memory; transport; conditioned taste aversion; calcium ion transport; regulation of neuron apoptosis; visual learning; negative regulation of neuron apoptosis; associative learning; cation transport; protein tetramerization; synaptic transmission, glutamatergic; neuromuscular process; response to amphetamine; social behavior; respiratory gaseous exchange; learning; pons maturation; memory; cellular calcium ion homeostasis; regulation of membrane potential; response to ethanol; olfactory learning; long-term memory; suckling behavior; propylene metabolic process; regulation of synaptogenesis; ionotropic glutamate receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; ion transport; cerebral cortex development; regulation of excitatory postsynaptic membrane potential; regulation of neuronal synaptic plasticity

0.025 mg

610 USD