RABBIT ANTI CONNEXIN 43 (pSer368) | MyBioSource MBS223268 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI CONNEXIN 43 (pSer368)

catalog :

MBS223268

quantity :

0.1 ml

price :

540 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, chicken, cow, zebrafish

application :

western blot

product information

catalog number :

MBS223268

products type :

Antibody

products full name :

RABBIT ANTI CONNEXIN 43 (pSer368)

products short name :

CONNEXIN 43

other names :

gap junction alpha-1 protein; Gap junction alpha-1 protein; gap junction alpha-1 protein; connexin 43; connexin-43; gap junction 43 kDa heart protein; gap junction membrane channel protein alpha 1; gap junction protein, alpha 1; Connexin-43; Cx43; Gap junction 43 kDa heart protein

other gene names :

Gja1; Gja1; Cx43; Cxn-43; Cx43

uniprot entry name :

CXA1_RAT

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Guinea Pig (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Zebrafish (Expected from Sequence)

sequence length :

382

specificity :

This item specifically recognises 43kDa Connexin43, when phosphorylated at Ser368. Connexin43 (CX43) allows for the exchange of solutions between neighbouring cells through the formation of gap-junctional channels and is expressed in the brain, kidneys and myocardium, as well as vascular endothelial cells. Studies suggest that a decrease in the permeability of gap-junctional channels occurs due to a conformational change in the structure of CX43, and that this is mediated by the phosphorylation of CX43 at residue Ser368, by Protein Kinase C (PKC). A possible link between CX43 and epilepsy has been investigated following the detection of high levels of CX43 in both low-grade tumours and peritumoural reactive astrocytes, see Aronica, E. et al. for details.

form :

Purified. Purified IgG - liquid

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.

tested application :

Western Blot (WB)*

app notes :

Western Blot: This item detects a band of approximately 43kDa in rat hippocampus cell lysates. Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. 0.01% Bovine Serum Albumin. 50% Glycerol

other info2 :

Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within connexin43 which includes phosphorylated Ser368. Buffer Solution: 10mM HEPES pH7.5. Antiserum Preparation: Antisera to rat connexin43 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography. Target Species: Rat

products description :

MBS223268 specifically recognises 43kDa Connexin43, when phosphorylated at Ser368. Connexin43 (CX43) allows for the exchange of solutions between neighbouring cells through the formation of gap-junctional channels and is expressed in the brain, kidneys and myocardium, as well as vascular endothelial cells. Studies suggest that a decrease in the permeability of gap-junctional channels occurs due to a conformational change in the structure of CX43, and that this is mediated by the phosphorylation of CX43 at residue Ser368, by Protein Kinase C (PKC). A possible link between CX43 and epilepsy has been investigated following the detection of high levels of CX43 in both low-grade tumours and peritumoural reactive astrocytes, see Aronica, E. et al. for details.

ncbi gi num :

6978896

ncbi acc num :

NP_036699.1

ncbi gb acc num :

NM_012567.2

uniprot acc num :

P08050

ncbi mol weight :

43,031 Da

ncbi pathways :

Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 117251!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 116129!!Calcium Regulation In The Cardiac Cell Pathway 198495!!EGFR1 Signaling Pathway 198427!!Formation Of Annular Gap Junctions Pathway 1122036!!Gap Junction Pathway 83464!!Gap Junction Pathway 483!!Gap Junction Assembly Pathway 1122030!!Gap Junction Degradation Pathway 1122035!!Gap Junction Trafficking Pathway 1122029

ncbi summary :

gap junction component; plays a role in cell-cell communication [RGD, Feb 2006]

size :

0.1 ml

price :

540 USD