antibody

RABBIT ANTI HUMAN ACTH

MBS223043

0.5 mL

375 USD

polyclonal

rabbit

nonconjugated

human, cow

ELISA, immunocytochemistry, enzyme immunoassay

Antibody

RABBIT ANTI HUMAN ACTH

[ACTH]

[ADRENOCORTICOTROPIC HORMONE]

[pro-opiomelanocortin preproprotein; Pro-opiomelanocortin; pro-opiomelanocortin; adrenocorticotropic hormone; adrenocorticotropin; alpha-MSH; alpha-melanocyte-stimulating hormone; beta-LPH; beta-MSH; beta-endorphin; beta-melanocyte-stimulating hormone; corticotropin-like intermediary peptide; corticotropin-lipotropin; gamma-LPH; gamma-MSH; lipotropin beta; lipotropin gamma; melanotropin alpha; melanotropin beta; melanotropin gamma; met-enkephalin; opiomelanocortin prepropeptide; pro-ACTH-endorphin; proopiomelanocortin preproprotein; proopiomelanocortin; Corticotropin-lipotropinCleaved into the following 11 chains:NPP; Melanotropin gammaAlternative name(s):Gamma-MSH]

[ACTH]

[POMC; POMC; LPH; MSH; NPP; POC; ACTH; CLIP; POMC; ACTH; CLIP]

COLI_HUMAN

Polyclonal

IgG

Rabbit

Reacts with: Rat. Based on sequence similarity, is expected to react with: Broad. N.B. Antibody reactivity and working conditions may vary between species.

267

SYSMEHFRWGKPVGKKRRPVKVYP

ACTH

Purified. Purified Ig - liquid

Approximate Protein Concentration: 5.0 mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen, ELISA (EIA), Immunofluorescence (IF), Immunohistology Paraffin

Immunohistology - Frozen: Minimum Dilution: 1/100; Maximum Dilution: 1/200;. ELISA: Minimum Dilution: 1/500; Maximum Dilution: 1/1000;. Immunohistology - Paraffin: Minimum Dilution: 1/100; Maximum Dilution: 1/200

Perservative Stabilisers: 0.09% Sodium Azide (NaN3). Preparation: Purified Ig prepared by affinity chromatography on Protein G

Histology Positive Control Tissue: ACTH-containing cells of the anterior pituitary gland. Buffer Solution: Phosphate buffered saline. Target Species: Human

Rabbit anti Human ACTH antibody recognizes human adrenocorticotrophic hormone, also known as corticotrophin. ACTH is a 4.5kDa hormone derived from cleavage of pro-opiomelanocortin (POMC), produced in the pituitary gland in response to corticotrophin releasing hormone (CRH) activity and functions to stimulate release of adrenal cortisol. Rabbit anti Human ACTH polyclonal antibody is directed against the N-terminal portion of adrenocorticotrophic hormone and also recognizes the ACTH cleavage fragment alpha-melanocyte stimulating hormone (alpha-MSH), responsible for melanin pigmentation control in most vertebrate species (Fiordelisio et al. 2006). Rabbit anti Human ACTH does not recognize the C-terminal ACTH cleavage product, corticotropin-like intermediary peptide (CLIP).

4505949

NP_000930.1

NM_000939.2

P01189

29,424 Da

Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Androgen Biosynthesis Pathway (106154); Biological Oxidations Pathway (105698); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Corticotropin-releasing Hormone Pathway (920957); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway (1127640); Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway (1127641)

This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Nov 2014]

POMC: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency and red hair. Belongs to the POMC family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2p23.3. Cellular Component: extracellular space; peroxisomal matrix; cytoplasm; extracellular region; peroxisome; secretory granule. Molecular Function: type 3 melanocortin receptor binding; G-protein-coupled receptor binding; type 4 melanocortin receptor binding; hormone activity; receptor binding. Biological Process: generation of precursor metabolites and energy; cellular protein metabolic process; cell-cell signaling; neuropeptide signaling pathway; regulation of blood pressure; peptide hormone processing; regulation of appetite; positive regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; signal transduction; glucose homeostasis; cellular pigmentation. Disease: Obesity; Proopiomelanocortin Deficiency

0.5 mL

375 USD