RABBIT ANTI RAT ALPHA SYNUCLEIN (pSer129) | MyBioSource MBS222961 product information

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product summary

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI RAT ALPHA SYNUCLEIN (pSer129)

catalog :

MBS222961

quantity :

0.1 ml

price :

705 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot

product information

catalog number :

MBS222961

products type :

Antibody

products full name :

RABBIT ANTI RAT ALPHA SYNUCLEIN (pSer129)

products short name :

ALPHA SYNUCLEIN

other names :

alpha-synuclein; Alpha-synuclein; alpha-synuclein; synuclein, alpha (non A4 component of amyloid precursor)

other gene names :

Snca; Snca

uniprot entry name :

SYUA_RAT

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Bovine (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Primate (Expected from Sequence)

sequence length :

140

specificity :

This item specifically recognises rat alpha synuclein when phosphorylated at Ser129. Alpha synuclein is a 140 amino acid, 15kDa protein seen predominantly in the neocortex, hippocampus, substantia nigra, thalamus and cerebellum. Its normal cellular function has not been definitively determined, but it is thought to be involved in the regulation of neuronal plasticity, dopamine neurotransmission and other membrane-associated processes at the presynaptic terminal. Alpha synuclein is normally a soluble, unfolded protein, but it can aggregate at high concentrations to form insoluble fibrils which, in association with other proteins such as ubiquitin, neurofilament protein and alpha B crystalline, form Lewy bodies. Lewy bodies are a hallmark pathological feature of Parkinson's disease and other neurodegenerative diseases. Alpha synuclein has several possible phosphorylation sites, Ser129 in particular can be phosphorylated by G-protein-coupled receptor kinases. Studies suggest that this phosphorylation promotes formation of alpha synuclein filaments, with hyperphosphorylation of Ser129 seen in Lewy bodies.

form :

Purified. Purified IgG - liquid

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Western Blot (WB)*

app notes :

Western Blot: This item detects a band of approximately 15kDa in rat cortex lysate. Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide (NaN3). 50% Glycerol. 0.01% Bovine Serum Albumin

other info2 :

Immunogen: Synthetic phosphopeptide corresponding to amino acid sequence surrounding phosphorylated serine 129 of Alpha Synuclein. Buffer Solution: 10mM Hepes pH7.5, 150mM NaCl. Antiserum Preparation: Antisera to Alpha Synulein (pSer129) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. Target Species: Rat

products description :

MBS222961 specifically recognises rat alpha synuclein when phosphorylated at Ser129. Alpha synuclein is a 140 amino acid, 15kDa protein seen predominantly in the neocortex, hippocampus, substantia nigra, thalamus and cerebellum. Its normal cellular function has not been definitively determined, but it is thought to be involved in the regulation of neuronal plasticity, dopamine neurotransmission and other membrane-associated processes at the presynaptic terminal. Alpha synuclein is normally a soluble, unfolded protein, but it can aggregate at high concentrations to form insoluble fibrils which, in association with other proteins such as ubiquitin, neurofilament protein and alpha B crystalline, form Lewy bodies. Lewy bodies are a hallmark pathological feature of Parkinson's disease and other neurodegenerative diseases. Alpha synuclein has several possible phosphorylation sites, Ser129 in particular can be phosphorylated by G-protein-coupled receptor kinases. Studies suggest that this phosphorylation promotes formation of alpha synuclein filaments, with hyperphosphorylation of Ser129 seen in Lewy bodies.

ncbi gi num :

9507125

ncbi acc num :

NP_062042.1

ncbi gb acc num :

NM_019169.2

uniprot acc num :

P37377

ncbi mol weight :

4,354 Da

ncbi pathways :

Alzheimer's Disease Pathway 83489!!Alzheimer's Disease Pathway 509!!Amyloids Pathway 1121861!!Disease Pathway 1121849!!EGFR1 Signaling Pathway 198427!!Parkinson's Disease Pathway 83490

ncbi summary :

may play a role in regulation of synaptic vesicle biogenesis and organization; may be involved in neurotransmission [RGD, Feb 2006]

size :

0.1 ml

price :

705 USD