RABBIT ANTI CARABIN (N-TERMINAL) | MyBioSource MBS222937 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI CARABIN (N-TERMINAL)

catalog :

MBS222937

quantity :

0.1 mg

price :

395 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS222937

products type :

Antibody

products full name :

RABBIT ANTI CARABIN (N-TERMINAL)

products short name :

CARABIN

other names :

carabin isoform 2; Carabin; carabin; TBC1 domain family, member 10C; TBC1 domain family member 10C

other gene names :

TBC1D10C; TBC1D10C; EPI64C; CARABIN

uniprot entry name :

TB10C_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse. N.B. Antibody reactivity and working conditions may vary between species.

sequence length :

310

specificity :

This item recognises human Carabin, also known as TBC1 domain family member 10C (TBC1D10C), a 50 kDa protein belonging to the TBC1 domain family of proteins. Carabin differs from other members of the TBC1 domain protein family, which appear to play a role in the regulation of cell growth and differentiation. Studies demonstrate that Carabin is part of a negative regulatory loop for the intracellular TCR signalling pathway and acts as an inhibitor of the Ras signalling pathway. Carabin is also believed to mediate crosstalk between calcineurin and Ras. Carabin is most abundantly expressed in spleen and peripheral blood leucocytes. This item does not cross react with either TBC1D10A or TBC1D10B.

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1.0mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Paraffin*, Western Blot (WB)

app notes :

Western Blot: This item detects a band of approximately 55kDa in human spleen tissue cell lysates. Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. . Western Blotting: Minimum Dilution: 1; Maximum Dilution: 2ug/ml

other info1 :

Perservative Stabilisers: 0.02% Sodium Azide (NaN3)

other info2 :

Immunogen: A 17 amino acid peptide from near the amino terminus of human Carabin. Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antiserum to human Carabin was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. Target Species: Human

products description :

MBS222937 recognises human Carabin, also known as TBC1 domain family member 10C (TBC1D10C), a 50 kDa protein belonging to the TBC1 domain family of proteins. Carabin differs from other members of the TBC1 domain protein family, which appear to play a role in the regulation of cell growth and differentiation. Studies demonstrate that Carabin is part of a negative regulatory loop for the intracellular TCR signalling pathway and acts as an inhibitor of the Ras signalling pathway. Carabin is also believed to mediate crosstalk between calcineurin and Ras. Carabin is most abundantly expressed in spleen and peripheral blood leucocytes. MBS222937 does not cross react with either TBC1D10A or TBC1D10B.

ncbi gi num :

374671768

ncbi acc num :

NP_001243437.1

ncbi gb acc num :

NM_001256508.1

uniprot acc num :

Q8IV04

ncbi mol weight :

32,887 Da

ncbi summary :

The protein encoded by this gene has an N-terminal Rab-GTPase domain and a binding site at the C-terminus for calcineurin, and is an inhibitor of both the Ras signaling pathway and calcineurin, a phosphatase regulated by calcium and calmodulin. Genes encoding similar proteins are located on chromosomes 16 and 22. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2013]

uniprot summary :

TBC1D10C: Inhibits the Ras signaling pathway through its intrinsic Ras GTPase-activating protein (GAP) activity. Acts as a negative feedback inhibitor of the calcineurin signaling pathway that also mediates crosstalk between calcineurin and Ras. Protein type: GAPs; GAPs, Rab. Chromosomal Location of Human Ortholog: 11q13.2. Cellular Component: filopodium membrane; membrane; plasma membrane. Biological Process: negative regulation of B cell activation; negative regulation of NFAT protein import into nucleus; retrograde transport, endosome to Golgi

size1 :

0.1 mg

price1 :

395 USD

more info or order :

MyBioSource product webpage