GOAT ANTI HUMAN Ku80 | MyBioSource MBS222869 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

GOAT ANTI HUMAN Ku80

catalog :

MBS222869

quantity :

0.1 mg

price :

515 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS222869

products type :

Antibody

products full name :

GOAT ANTI HUMAN Ku80

products short name :

Ku80

other names :

X-ray repair cross-complementing protein 5; X-ray repair cross-complementing protein 5; X-ray repair cross-complementing protein 5; 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku autoantigen, 80kDa; Ku86 autoantigen related protein 1; TLAA; lupus Ku autoantigen protein p86; nuclear factor IV; thyroid-lupus autoantigen; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku80; Ku86; Lupus Ku autoantigen protein p86; Nuclear factor IV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)

products gene name :

Ku80

other gene names :

XRCC5; XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1; G22P2; CTC85; CTCBF; TLAA

uniprot entry name :

XRCC5_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Goat

sequence length :

732

specificity :

This item specifically recognises the 80kDa subunit of human Ku protein, an evolutionarily conserved nuclear ATP-dependent DNA helicase, involved in a major proportion of DNA repair and in V(D)J recombination. The Ku protein, originally described as an autoantigen, exists as a tightly associated heterodimer consisting of a 70kDa (Ku70) and 80kDa (Ku80) subunit which binds to DNA double-strand break ends (DSB). DNA bound Ku recruits the large catalytic subunit DNA-PKcs to form the DNA-dependent protein kinase complex DNA-PK, facilitating DNA repair by the non-homologous end-joining (NHEJ) pathway.

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 0.5mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

ELISA (EIA), Western Blot (WB)

app notes :

Western Blot: This item detects a band of approximately 80-85kDa in HeLa cell lysates. An additional 75kDa band of unknown origin may be observed. ELISA: Maximum Dilution: 1/16,000. Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.3ug/ml

other info1 :

Perservative Stabilisers: 0.02% Sodium Azide (NaN3). 0.5% Bovine Serum Albumin

other info2 :

Immunogen: Peptide sequence C-SLAKKDEKTDTLED from the internal region of Ku80 (NP_066964.1). Buffer Solution: TRIS buffered saline. Antiserum Preparation: Antiserum to human Ku80 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography. Target Species: Human

products description :

MBS222869 specifically recognises the 80kDa subunit of human Ku protein, an evolutionarily conserved nuclear ATP-dependent DNA helicase, involved in a major proportion of DNA repair and in V(D)J recombination. The Ku protein, originally described as an autoantigen, exists as a tightly associated heterodimer consisting of a 70kDa (Ku70) and 80kDa (Ku80) subunit which binds to DNA double-strand break ends (DSB). DNA bound Ku recruits the large catalytic subunit DNA-PKcs to form the DNA-dependent protein kinase complex DNA-PK, facilitating DNA repair by the non-homologous end-joining (NHEJ) pathway.

ncbi gi num :

10863945

ncbi acc num :

NP_066964.1

ncbi gb acc num :

NM_021141.3

uniprot acc num :

P13010

ncbi mol weight :

82,705 Da

ncbi pathways :

2-LTR Circle Formation Pathway (105993); BARD1 Signaling Events Pathway (137959); Coregulation Of Androgen Receptor Activity Pathway (138085); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); DNA Repair Pathway (105837); DNA-PK Complex Pathway (413430); DNA-PK Complex Pathway (890556); Disease Pathway (530764); Double-Strand Break Repair Pathway (105861); Early Phase Of HIV Life Cycle Pathway (105985)

ncbi summary :

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]

uniprot summary :

Ku80: the 80-kilodalton subunit of the Ku complex, also known as ATP-dependant DNA helicase II. A single stranded DNA-dependent ATP-dependent helicase. It functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3 -5 direction. Binding to DNA may be mediated by p70. Protein type: EC 3.6.4.-; EC 3.6.1.-; DNA-binding; Nucleolus; RNA-binding; Nuclear receptor co-regulator; Helicase. Chromosomal Location of Human Ortholog: 2q35. Cellular Component: nucleoplasm; nuclear telomere cap complex; nuclear chromosome, telomeric region; membrane; nucleolus; plasma membrane; cytosol; nucleus. Molecular Function: protein C-terminus binding; ATP-dependent DNA helicase activity; protein binding; DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; damaged DNA binding; telomeric DNA binding; double-stranded telomeric DNA binding; 5 -deoxyribose-5-phosphate lyase activity; ATP binding. Biological Process: response to drug; positive regulation of neurogenesis; transcription, DNA-dependent; viral reproduction; DNA repair; double-strand break repair via nonhomologous end joining; DNA duplex unwinding; DNA recombination; cell proliferation; double-strand break repair; positive regulation of interferon type I production; innate immune response; brain development; negative regulation of transcription, DNA-dependent; telomere maintenance

size1 :

0.1 mg

price1 :

515 USD

more info or order :

MyBioSource product webpage