RABBIT ANTI PLK1 (pThr210) | MyBioSource MBS222787 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI PLK1 (pThr210)

catalog :

MBS222787

quantity :

0.1 ml

price :

455 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow, zebrafish

application :

western blot

product information

catalog number :

MBS222787

products type :

Antibody

products full name :

RABBIT ANTI PLK1 (pThr210)

products short name :

PLK1

other names :

serine/threonine-protein kinase PLK1; Serine/threonine-protein kinase PLK1; serine/threonine-protein kinase PLK1; PLK-1; polo-like kinase homolog; polo-like kinase 1; Polo-like kinase 1; PLK-1

products gene name :

PLK1

other gene names :

Plk1; Plk1; Plk; Plk; PLK-1

uniprot entry name :

PLK1_RAT

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Bovine (Expected from Sequence), Dog (Expected from Sequence), Human, Mouse (Expected from Sequence), Primate (Expected from Sequence), Xenopus (Expected from Sequence), Zebrafish (Expected from Sequence)

sequence length :

603

specificity :

This item specifically recognises the 66kDa serine/threonine kinase, PLK1 (polo-like kinase 1), when phosphorylated at Thr210. PLK1 is a member of a group of polo-like kinases which play essential roles in the progression of mitosis. PLK1 has been implicated in various stages of mitosis, including promotion through the activation of cyclin B1/Cdk (mitosis promoting factor), the formation of functional mitotic bipolar spindles, and the maturation of centrosomes. The timing at which PLK1 phosphorylation occurs at Ser137 and Thr210 sites, may act as a regulator of PLK1 function through the mitotic cycle.

form :

Purified. Purified IgG - liquid

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.

tested application :

Western Blot (WB)*

app notes :

Western Blot: This item detects a band of approximately 66kDa in rat synaptic membrane cell lysates. Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide (NaN3). 0.01% Bovine Serum Albumin. 50% Glycerol

other info2 :

Immunogen: Synthetic phospeptide corresponding to an amino acid sequence within PLK1, which includes phosphorylated Thr210. Buffer Solution: 10mM HEPES pH7.5. Antiserum Preparation: Antisera to PLK1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography. Target Species: Rat

products description :

MBS222787 specifically recognises the 66kDa serine/threonine kinase, PLK1 (polo-like kinase 1), when phosphorylated at Thr210. PLK1 is a member of a group of polo-like kinases which play essential roles in the progression of mitosis. PLK1 has been implicated in various stages of mitosis, including promotion through the activation of cyclin B1/Cdk (mitosis promoting factor), the formation of functional mitotic bipolar spindles, and the maturation of centrosomes. The timing at which PLK1 phosphorylation occurs at Ser137 and Thr210 sites, may act as a regulator of PLK1 function through the mitotic cycle.

ncbi gi num :

25742783

ncbi acc num :

NP_058796.1

ncbi gb acc num :

NM_017100.1

uniprot acc num :

Q62673

ncbi mol weight :

68,291 Da

ncbi pathways :

APC/C-mediated Degradation Of Cell Cycle Proteins Pathway 1121710!!APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway 1121723!!Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway 1121714!!Activation Of NIMA Kinases NEK9, NEK6, NEK7 Pathway 1121694!!Anchoring Of The Basal Body To The Plasma Membrane Pathway 1121325!!Assembly Of The Primary Cilium Pathway 1121324!!Cell Cycle Pathway 1121638!!Cell Cycle, Mitotic Pathway 1121658!!Cell Cycle Pathway 198428!!Cell Cycle Pathway 83446

ncbi summary :

human homolog catalyzes the phosphorylation of a Golgi reassembly stacking protein (GRASP65); may play a role in Golgi apparatus fragmentation and reorganization during mitosis [RGD, Feb 2006]

size :

0.1 ml

price :

455 USD