antibody

RABBIT ANTI RAT NMDA RECEPTOR NR2B (pSer1480)

MBS222784

0.1 mL

455 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, dog, chicken, cow, zebrafish

Antibody

RABBIT ANTI RAT NMDA RECEPTOR NR2B (pSer1480)

NMDAR NR2B

glutamate receptor ionotropic, NMDA 2B; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor ionotropic, NMDA 2B; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B; glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor, ionotropic, NMDA2B; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B

NMDA

Grin2b; Grin2b; GluN2B; GluN2B; NMDAR2B; NR2B

NMDE2_RAT

Polyclonal

IgG

Rabbit

Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Primate (Expected from Sequence), Zebrafish (Expected from Sequence)

1482

This item specifically recognises the 180kDa NR2B subunit of the NMDA (N-methyl-D-aspartate) receptor, when phosphorylated at Ser1480.Receptors for NMDA belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Several antagonists and agonists of NMDA receptors (NMDAR) have been identified, including the glutamate analogue homoquinolinic acid, which displays a higher affinity for NR2B-containing NMDAR. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+permeability. Studies have shown that the phosphorylation of the Ser1480 site within the C-terminal PDZ ligand of the NR2B subunit of NMDAR, by casein kinase II (CK2), results in a decrease in neuron NR2B surface expression, see Chung, H.J. et al. for details.The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer's disease (Popke, E.J.) and also epilepsy and ischemic neuronal cell death.

Purified. Purified IgG - liquid

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.

Western Blot (WB)*

Western Blot: This item detects a band of approximately 180kDa in rat hippocampal cell lysates. Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

Perservative Stabilisers: 0.09% Sodium Azide (NaN3). 0.01% Bovine Serum Albumin. 50% Glycerol

Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within the NR2B subunit of the rat NMDA receptor, which includes phoshorylated Ser1480. Buffer Solution: 10mM Hepes pH7.5. Antiserum Preparation: Antisera to rat NMDA receptor NR2B subunit were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography. Target Species: Rat

MBS222784 specifically recognises the 180kDa NR2B subunit of the NMDA (N-methyl-D-aspartate) receptor, when phosphorylated at Ser1480. Receptors for NMDA belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Several antagonists and agonists of NMDA receptors (NMDAR) have been identified, including the glutamate analogue homoquinolinic acid, which displays a higher affinity for NR2B-containing NMDAR. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+permeability. Studies have shown that the phosphorylation of the Ser1480 site within the C-terminal PDZ ligand of the NR2B subunit of NMDAR, by casein kinase II (CK2), results in a decrease in neuron NR2B surface expression, see Chung, H.J. et al. for details. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer's disease (Popke, E.J.) and also epilepsy and ischemic neuronal cell death.

6980984

NP_036706.1

NM_012574.1

Q00960

166,071 Da

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (1121188); Alcoholism Pathway (585585); Alcoholism Pathway (587116); Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Amphetamine Addiction Pathway (547642); Amphetamine Addiction Pathway (550546); Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Axon Guidance Pathway (1121351)

subunit of the NMDA receptor glutamate gated ion channel; displays voltage dependent sensitivity to magnesium, calcium permeability, and glycine regulated activity; may facilitate learning and long term potentiation [RGD, Feb 2006]

NMDAR2B: an NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. Protein type: Channel, ligand-gated; Channel, calcium; Membrane protein, multi-pass; Membrane protein, integral. Cellular Component: neuron projection; cell surface; postsynaptic density; dendritic spine; terminal button; Z disc; N-methyl-D-aspartate selective glutamate receptor complex; presynaptic membrane; postsynaptic membrane; synaptic vesicle; membrane; synapse; cell junction. Molecular Function: neurotransmitter binding; interleukin-1 receptor binding; zinc ion binding; calcium channel activity; beta-catenin binding; drug binding; ionotropic glutamate receptor binding; protein binding; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; protein heterodimerization activity; glycine binding; N-methyl-D-aspartate selective glutamate receptor activity; cation channel activity; cell adhesion molecule binding; receptor binding; D2 dopamine receptor binding. Biological Process: regulation of long-term neuronal synaptic plasticity; fear response; behavioral fear response; startle response; response to toxin; positive regulation of glutamate secretion; rhythmic process; regulation of synaptic plasticity; response to other organism; receptor clustering; response to organic cyclic substance; positive regulation of synaptic transmission; response to carbohydrate stimulus; synaptic transmission; behavioral response to pain; response to magnesium ion; learning and/or memory; calcium ion transport; response to methylmercury; response to electrical stimulus; cation transport; associative learning; cellular response to magnesium starvation; synaptic transmission, glutamatergic; regulation of action potential; in utero embryonic development; hippocampus development; response to amphetamine; learning; response to cocaine; memory; response to manganese ion; detection of mechanical stimulus involved in sensory perception of pain; regulation of postsynaptic membrane potential; response to ethanol; sensory organ development; long-term memory; suckling behavior; response to mechanical stimulus; ionotropic glutamate receptor signaling pathway; regulation of MAPKKK cascade; response to cytokine stimulus; cerebral cortex development; regulation of excitatory postsynaptic membrane potential; response to calcium ion; multicellular organismal response to stress; response to amine stimulus

0.1 mL

455 USD