antibody

RABBIT ANTI RAT MEK 5 (pSer311/Thr315)

MBS222783

0.1 mL

450 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, dog, chicken, cow, zebrafish

Antibody

RABBIT ANTI RAT MEK 5 (pSer311/Thr315)

MEK 5

dual specificity mitogen-activated protein kinase kinase 5 isoform a; Dual specificity mitogen-activated protein kinase kinase 5; dual specificity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5; MAPK/ERK kinase 5; MAPKK 5; MEK 5; mitogen activated protein kinase kinase 5; MAPK/ERK kinase 5; MEK 5

MEK 5

Map2k5; Map2k5; Mek5; Mek5; Mkk5; Prkmk5; MAP kinase kinase 5; MAPKK 5; MEK 5

MP2K5_RAT

Polyclonal

IgG

Rabbit

Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Primate (Expected from Sequence), Xenopus (Expected from Sequence), Zebrafish (Expected from S

448

This item specifically recognises 49kDa MEK5, also known as MAP2K5, when phosphorylated at Ser311 and Thr315. MEK5 is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family, which acts as a key regulator in a mitogen-activated protein (MAP) kinase cascade, and hence growth factor-induced cellular stimulation. MEK5 exists in naturally occurring MEK5alpha and MEK5beta splice variants, the former of which is known to specifically activate/regulate MAPK7/ERK5/BMK1, whilst the latter may act as a suppressor of MAPK7 activation. Activation of MEK5 itself is regulated by upstream MAP3K3/MEKK3, and phosphorylation of MEK5 at both the Ser311 and Thr315 sites has been implicated in cell proliferation.

Purified. Purified IgG - liquid

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.

Western Blot (WB)*

Western Blot: This item detects a band of approximately 49kDa in rat testes cell lysates. Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

Perservative Stabilisers: 0.09% Sodium Azide (NaN3). 0.01% Bovine Serum Albumin. 50% Glycerol

Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within MEK5, which includes phoshorylated Ser311 and Thr315. Buffer Solution: 10mM Hepes pH7.5. Antiserum Preparation: Antisera to MEK5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography. Target Species: Rat

MBS222783 specifically recognises 49kDa MEK5, also known as MAP2K5, when phosphorylated at Ser311 and Thr315. MEK5 is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family, which acts as a key regulator in a mitogen-activated protein (MAP) kinase cascade, and hence growth factor-induced cellular stimulation. MEK5 exists in naturally occurring MEK5alpha and MEK5beta splice variants, the former of which is known to specifically activate/regulate MAPK7/ERK5/BMK1, whilst the latter may act as a suppressor of MAPK7 activation. Activation of MEK5 itself is regulated by upstream MAP3K3/MEKK3, and phosphorylation of MEK5 at both the Ser311 and Thr315 sites has been implicated in cell proliferation.

77157802

NP_001029159.1

NM_001033987.1

Q62862

40,091 Da

EGFR1 Signaling Pathway (198427); Gap Junction Pathway (83464); Gap Junction Pathway (483); Insulin Signaling Pathway (198452); Integrin-mediated Cell Adhesion Pathway (198487); MAPK (ERK5) Signaling Pathway (1085055); MAPK (ERK5) Signaling Pathway (1108229); MAPK Signaling Pathway (198496); MAPK Signaling Pathway (83440); MAPK Signaling Pathway (456)

kinase involved in signal transduction in the cell [RGD, Feb 2006]

MEK5: a dual specificity protein kinase of the STE7 family. Activates ERK5 and is itself phosphorylated and activated by MEKK3, as well as by atypical protein kinase C isoforms. Involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced isoforms have been described. Protein type: Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); EC 2.7.12.2; STE group; STE7 family. Cellular Component: membrane; spindle; intracellular; cytosol; nucleus. Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; metal ion binding; protein-tyrosine kinase activity; ATP binding; protein kinase activity. Biological Process: peptidyl-tyrosine phosphorylation; negative regulation of heterotypic cell-cell adhesion; activation of MAPK activity; heart development; MAPKKK cascade; negative regulation of interleukin-8 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; positive regulation of cell growth; protein amino acid phosphorylation; positive regulation of protein metabolic process; inhibition of NF-kappaB transcription factor; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation

0.1 mL

450 USD