RABBIT ANTI HUMAN CTGF | MyBioSource MBS222751 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI HUMAN CTGF

catalog :

MBS222751

quantity :

0.1 mg

price :

540 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS222751

products type :

Antibody

products full name :

RABBIT ANTI HUMAN CTGF

products short name :

CTGF

other names :

connective tissue growth factor; Connective tissue growth factor; connective tissue growth factor; CCN family member 2; IBP-8; IGF-binding protein 8; IGFBP-8; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8; connective tissue growth factor; CCN family member 2; Hypertrophic chondrocyte-specific protein 24; Insulin-like growth factor-binding protein 8; IBP-8; IGF-binding protein 8; IGFBP-8

products gene name :

CTGF

other gene names :

CTGF; CTGF; CCN2; NOV2; HCS24; IGFBP8; CCN2; HCS24; IGFBP8; IBP-8; IGF-binding protein 8; IGFBP-8

uniprot entry name :

CTGF_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

sequence length :

349

form :

Purified. Purified IgG - lyophilised

concentration :

IgG concentration 1.0mg/ml after reconstitution.

storage stability :

Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of reconstitution.

tested application :

ELISA (EIA), Functional Assays (FN), Immunofluorescence (IF), Immunohistology Paraffin*, Western Blot (WB)

app notes :

ELISA: This purified human CTGF antibody may ne used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human CTGF antibody as the detection reagent and recombinant human CTGF as the standard. ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml. Functional Assays: Minimum Dilution: 6.0; Maximum Dilution: 9.0ug/ml. Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. . Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml

other info1 :

Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.

other info2 :

Immunogen: Recombinant human CTGF. Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to human CTGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. Target Species: Human

products description :

Rabbit anti Human CTGF antibody specifically recognizes human CTGF (connective tissue growth factor), a 323 amino acid polypeptide and member of the CCN family of secreted cysteine rich regulatory proteins, which is produced by vascular endothelial and umbilical vein cells, and interacts with several other growth factors, including transforming growth factor beta (TGF-beta), vascular endothelial growth factor (VEGF) and bone morphogenetic proteins (BMPs). CTGF is a heparin-binding pleiotropic growth factor involved in chondrogenesis and potentially skeletogenesis. CTGF promotes the adhesion of epithelial cells and fibroblasts, stimulates integrin expression, and also promotes endothelial cell survival, migration and adhesion, implicating CTGF in angiogenesis. CTGF induces extracellular matrix formation, including the deposition of excessive collagen in fibrotic diseases, and has also been implicated in wound healing.

ncbi gi num :

4503123

ncbi acc num :

NP_001892.1

ncbi gb acc num :

NM_001901.2

uniprot acc num :

P29279

ncbi mol weight :

35,205 Da

ncbi pathways :

Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPARA Activates Gene Expression Pathway (530749); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (119544); TGF-beta Receptor Signaling Pathway (138024)

ncbi summary :

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]

uniprot summary :

CTGF: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Cell adhesion; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 6q23.1. Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; cis-Golgi network; intracellular membrane-bound organelle; perinuclear region of cytoplasm; extracellular region; plasma membrane; cell cortex; cytosol. Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; protein binding; growth factor activity; fibronectin binding. Biological Process: response to peptide hormone stimulus; epidermis development; response to anoxia; cell-matrix adhesion; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; positive regulation of caspase activity; cellular lipid metabolic process; response to estradiol stimulus; cell-cell signaling; positive regulation of cell proliferation; positive regulation of stress fiber formation; tissue homeostasis; response to glucose stimulus; response to wounding; regulation of cell growth; angiogenesis; cell adhesion; cell differentiation; positive regulation of cell activation; cartilage condensation; integrin-mediated signaling pathway; transcription initiation from RNA polymerase II promoter; cell migration; ossification; fibroblast growth factor receptor signaling pathway; organ senescence; response to mineralocorticoid stimulus; response to amino acid stimulus; gene expression; regulation of chondrocyte differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of cell differentiation; lung development

size1 :

0.1 mg

price1 :

540 USD

more info or order :

MyBioSource product webpage