catalog number :
MBS222660
products full name :
SHEEP ANTI HUMAN FIBRINOGEN:FITC
products short name :
FIBRINOGEN
other names :
fibrinogen alpha chain isoform alpha-E preproprotein; Fibrinogen alpha chain; fibrinogen alpha chain; fibrinogen, A alpha polypeptide; fibrinogen alpha chain
other gene names :
FGA; FGA; Fib2
uniprot entry name :
FIBA_HUMAN
form :
FITC. Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
concentration :
IgG concentration 1.0mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Immunofluorescence (IF)
app notes :
Immunofluorescence: Minimum Dilution: 1/10; Maximum Dilution: 1/100
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
other info2 :
Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to human fibrinogen were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. Target Species: Human
products description :
Sheep anti Human fibrinogen antibody recognizes human fibrinogen, a complex ~340kDa hetero-hexameric (di-trimeric) glycoprotein consisting of 3 pairs of alpha, beta and gamma chains linked by a series of 29 disulphide bonds (Henschen et al. 1983). The six chains are arranged in such a way that all the N-Terminal ends adjoin to form a central E domain with two trimeric coiled coil structures connecting to outer D domains. Fibrinogen plays an important role in the coagulation process with the D and E domains interacting via the C-Terminal ends of the alpha chains during fibrin clot cross-linking. Sheep anti human fibrinogen antibody shows minimal cross-reactivity with related serum proteins. Fibrinogen has been identified as a ferritin binding protein in the horse (Orino et al. 1993). Sheep anti human fibrinogen antibody has been successfully as a capture reagent for ferritin - anti ferritin IgG complexes in horse plasma to evaluate the antibody response to ferritin by ELISA (Takahashi et al. 2013).
ncbi acc num :
NP_000499.1
ncbi gb acc num :
NM_000508.3
ncbi mol weight :
69,757 Da
ncbi pathways :
Amyloids Pathway 366238!!Blood Clotting Cascade Pathway 198840!!Common Pathway 106060!!Complement And Coagulation Cascades Pathway 83073!!Complement And Coagulation Cascades Pathway 484!!Disease Pathway 530764!!Extracellular Matrix Organization Pathway 576262!!Formation Of Fibrin Clot (Clotting Cascade) Pathway 106057!!GRB2:SOS Provides Linkage To MAPK Signaling For Integrins Pathway 106055!!Hemostasis Pathway 106028
ncbi summary :
The protein encoded by this gene is the alpha component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in two isoforms which vary in the carboxy-terminus. [provided by RefSeq, Jul 2008]