antibody

RABBIT ANTI HUMAN NFkB p50

MBS222358

0.1 mL

705 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, immunoprecipitation, enzyme immunoassay, EMSA

Antibody

RABBIT ANTI HUMAN NFkB p50

NFkB p50

nuclear factor NF-kappa-B p105 subunit isoform 2; Nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p105 subunit; DNA binding factor KBF1; DNA-binding factor KBF1; NF-kappabeta; nuclear factor NF-kappa-B p50 subunit; nuclear factor kappa-B DNA binding subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1Nuclear factor NF-kappa-B p50 subunit

NFkB p50

NFKB1; NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

NFKB1_HUMAN

Polyclonal

IgG

Rabbit

968

Serum. Serum - liquid

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

ELISA (EIA), Gel Super Shift Assays (GS), Immunoprecipitation (IP), Western Blot (WB)

Gel Super Shift Assays: Maximum Dilution: 0.5 - 1ul per test;. Western Blotting: Maximum Dilution: 1/1000

Perservative Stabilisers: 0.01% Sodium Azide

Immunogen: Human NFkB p50 peptide (N-terminus) conjugated to KLH. Antiserum Preparation: Antisera to NFkB p50 were raised by repeated immunisation of rabbits with highly purified antigen. Target Species: Human

Rabbit anti Human NFkB p50 antibody recognizes human NFkB p50, and also binds to the p105 precursor protein. In gel supershift assays the antiserum shows activity against both p50:p50 homodimers and p50:p65 heterodimers.

259155302

NP_001158884.1

NM_001165412.1

P19838

85,520 Da

AGE/RAGE Pathway (698754); Activated TLR4 Signalling Pathway (106400); Activation Of NF-kappaB In B Cells Pathway (576251); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (366160); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Alternative NF-kappaB Pathway (138004); Amoebiasis Pathway (167324)

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

NFkB-p105: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two alternatively spliced isoforms have been described. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 4q24. Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus; cytosol. Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; heat shock protein binding; double-stranded DNA binding; chromatin binding; transcription factor activity; transcription factor binding. Biological Process: transcription from RNA polymerase II promoter; I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of cholesterol transport; negative regulation of cellular protein metabolic process; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of interferon type I production; inflammatory response; toll-like receptor 4 signaling pathway; membrane protein intracellular domain proteolysis; MyD88-independent toll-like receptor signaling pathway; negative regulation of interleukin-12 biosynthetic process; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to copper ion; negative regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; response to oxidative stress; negative regulation of apoptosis

0.1 mL

705 USD