antibody

GOAT ANTI 4-HYDROXYNONENAL

MBS222342

0.1 mL

405 USD

polyclonal

goat

nonconjugated

western blot, ELISA, enzyme immunoassay

Antibody

GOAT ANTI 4-HYDROXYNONENAL

4-HYDROXYNONENAL

Polyclonal

IgG

Goat

This item recognises 4-hydroxynonenal (HNE). A 65 kDa, highly reactive aldehyde released upon oxidation of omega-6-insaturated fatty acids, often by free radicals. HNE acts to spread and increase the initial effects of free radical events. It binds nucleic acids, phospholipids and sulfhydryl, histidine and lysine groups. These conjugates cause the cytotoxic effects (including cell death) that occur during oxidative stress due to hydrogen peroxide, superoxide, UV, heat and oxidant chemicals. HNE plays a role in the pathogenesis of diseases, stimulating fibrogenesis and inflammation. It is thought to act as a sensor of external stimuli, inducing the stress response by modulating membrane receptors such as Epidermal growth factor receptor or Fas. It is thought that constitutive levels of HNE may be needed for normal cell functions as decreased HNE levels are associated with cell proliferation and increased HNE levels with elevated apoptosis.

Serum. Serum - liquid

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

ELISA (EIA), Western Blot (WB)

ELISA: Maximum Dilution: 1/10000;. Western Blotting: Maximum Dilution: 1/3000

Perservative Stabilisers: 0.1% Sodium Azide (NaN3)

Immunogen: 4-hydroxynonenal conjugate. Antiserum Preparation: Antisera to 4-hydroxynonenal were raised by repeated immunisations of goats with highly purified antigen. Target Species: Broad

MBS222342 recognises 4-hydroxynonenal (HNE). A 65 kDa, highly reactive aldehyde released upon oxidation of omega-6-insaturated fatty acids, often by free radicals. HNE acts to spread and increase the initial effects of free radical events. It binds nucleic acids, phospholipids and sulfhydryl, histidine and lysine groups. These conjugates cause the cytotoxic effects (including cell death) that occur during oxidative stress due to hydrogen peroxide, superoxide, UV, heat and oxidant chemicals. HNE plays a role in the pathogenesis of diseases, stimulating fibrogenesis and inflammation. It is thought to act as a sensor of external stimuli, inducing the stress response by modulating membrane receptors such as Epidermal growth factor receptor or Fas. It is thought that constitutive levels of HNE may be needed for normal cell functions as decreased HNE levels are associated with cell proliferation and increased HNE levels with elevated apoptosis.

0.1 mL

405 USD