RABBIT ANTI HUMAN p27/kip1 | MyBioSource MBS222339 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI HUMAN p27/kip1

catalog :

MBS222339

quantity :

0.05 mL

price :

395 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunoprecipitation, enzyme immunoassay

product information

catalog number :

MBS222339

products type :

Antibody

products full name :

RABBIT ANTI HUMAN p27/kip1

products short name :

p27/kip1

other names :

cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; p27Kip1

products gene name :

p27/kip1

other gene names :

CDKN1B; CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1; KIP1

uniprot entry name :

CDN1B_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

sequence length :

198

specificity :

This item detects p27, a member of the Kip/Cip family of proteins which is also known as p27kip1. p27 is a cyclin dependent kinase inhibitor (CKI) which controls G1/S phase transition in the cell cycle. p27 prevents cell cycle progression by inhibiting the activity of cyclin E-Cdk2 and cyclin D-Cdk4/6, thus preventing E2F activation and S phase progression. Low p27 levels and/or cytosolic mis-localisation have been associated with the development of lung, breast, colon, oesophagus, and prostate tumours.

form :

Serum. Serum - liquid

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)

app notes :

Western Blotting: Maximum Dilution: 1/2000

other info1 :

Perservative Stabilisers: 0.01% Sodium Azide (NaN3)

other info2 :

Immunogen: Full length human p27 protein. Antiserum Preparation: Antisera to p27/kip1 were raised by repeated immunisations of rabbits with highly purified antigen. Target Species: Human

products description :

MBS222339 detects p27, a member of the Kip/Cip family of proteins which is also known as p27kip1. p27 is a cyclin dependent kinase inhibitor (CKI) which controls G1/S phase transition in the cell cycle. p27 prevents cell cycle progression by inhibiting the activity of cyclin E-Cdk2 and cyclin D-Cdk4/6, thus preventing E2F activation and S phase progression. Low p27 levels and/or cytosolic mis-localisation have been associated with the development of lung, breast, colon, oesophagus, and prostate tumours.

ncbi gi num :

4757962

ncbi acc num :

NP_004055.1

ncbi gb acc num :

NM_004064.4

uniprot acc num :

P46527

ncbi mol weight :

22,073 Da

ncbi pathways :

AKT Phosphorylates Targets In The Cytosol Pathway (106475); Adaptive Immune System Pathway (366160); AhR Pathway (755436); C-MYB Transcription Factor Network Pathway (138073); Cell Cycle Pathway (530733); Cell Cycle Checkpoints Pathway (105739); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463)

ncbi summary :

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]

uniprot summary :

p27Kip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. May mediate TGF beta-induced g1 arrest. Its degradation is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Protein type: Oncoprotein; Inhibitor; Protein kinase, regulatory subunit; Cell cycle regulation. Chromosomal Location of Human Ortholog: 12p13.1-p12. Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus; endosome. Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; chaperone binding; protein complex binding; caspase activator activity; Hsp70 protein binding; protein phosphatase binding; transforming growth factor beta receptor, cytoplasmic mediator activity. Biological Process: negative regulation of kinase activity; response to peptide hormone stimulus; nerve growth factor receptor signaling pathway; positive regulation of microtubule polymerization; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; response to estradiol stimulus; negative regulation of cell proliferation; sensory perception of sound; positive regulation of cell proliferation; response to glucose stimulus; negative regulation of mitotic cell cycle; cell cycle arrest; inner ear development; potassium ion transport; epidermal growth factor receptor signaling pathway; caspase activation; response to drug; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; negative regulation of cyclin-dependent protein kinase activity; negative regulation of cell motility; response to amino acid stimulus; response to cadmium ion; autophagic cell death; positive regulation of protein catabolic process; response to hypoxia; innate immune response; negative regulation of phosphorylation; negative regulation of cell growth; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; negative regulation of transcription, DNA-dependent; G1/S transition of mitotic cell cycle; negative regulation of apoptosis. Disease: Multiple Endocrine Neoplasia, Type Iv

size1 :

0.05 mL

price1 :

395 USD