antibody

RABBIT ANTI CYCLIN E

MBS222338

0.05 mL

390 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunoprecipitation, enzyme immunoassay

Antibody

RABBIT ANTI CYCLIN E

CYCLIN E

G1/S-specific cyclin-E1; G1/S-specific cyclin-E1; G1/S-specific cyclin-E1; cyclin E variant ex5del; cyclin E variant ex7del; cyclin Es; cyclin Et; cyclin E1

CYCLIN E

CCNE1; CCNE1; CCNE; CCNE

CCNE1_HUMAN

Polyclonal

IgG

Rabbit

Mouse, Rat

410

This item detects cyclin E, a nuclear protein which is the regulatory subunit of cyclin dependent kinase 2 (Cdk2), and controls S phase entry in cells. Cyclin E follows a cyclic pattern of expression during the cell cycle, with highest expression at the G1-S boundary. Although cyclin E has an important physiological role in normal cell division, recent reports suggest this function is redundant, and its key functions occur as cells exit quiescence, and in endoreduplication. Cyclin E is tightly regulated during normal cell cycles, but is often deregulated in carcinomas, lymphoma, and leukaemia.

Serum. Serum - liquid

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)

Western Blot: This item detects a band of approximately 45kDa in from human, rat and mouse tissue. ELISA: Maximum Dilution: 1/2000. Western Blotting: Minimum Dilution: 1/200; Maximum Dilution: 1/500

Perservative Stabilisers: 0.01% Sodium Azide (NaN3)

Immunogen: Full length synthetic polypeptide corresponding to the human gene sequence of cyclin E. Antiserum Preparation: Antisera to cyclin E were raised by repeated immunisations of rabbits with highly purified antigen. Target Species: Human

MBS222338 detects cyclin E, a nuclear protein which is the regulatory subunit of cyclin dependent kinase 2 (Cdk2), and controls S phase entry in cells. Cyclin E follows a cyclic pattern of expression during the cell cycle, with highest expression at the G1-S boundary. Although cyclin E has an important physiological role in normal cell division, recent reports suggest this function is redundant, and its key functions occur as cells exit quiescence, and in endoreduplication. Cyclin E is tightly regulated during normal cell cycles, but is often deregulated in carcinomas, lymphoma, and leukaemia.

17318559

NP_001229.1

NM_001238.2

P24864

45,150 Da

Androgen Receptor Signaling Pathway (198806); B Cell Receptor Signaling Pathway (198909); BARD1 Signaling Events Pathway (137959); Cell Cycle Pathway (530733); Cell Cycle Checkpoints Pathway (105739); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Cell Cycle - G1/S Transition Pathway (1084768)

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq, Jul 2008]

CCNE1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Two alternatively spliced isoforms have been described. Protein type: Cell cycle regulation; Activator; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 19q12. Cellular Component: nucleoplasm; cyclin-dependent protein kinase holoenzyme complex; cytosol; nucleus. Molecular Function: protein binding; androgen receptor binding; transcription coactivator activity; kinase activity; cyclin-dependent protein kinase regulator activity; protein kinase binding. Biological Process: G1/S-specific transcription in mitotic cell cycle; Wnt receptor signaling pathway; DNA replication initiation; cell division; regulation of cell cycle; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; mitotic cell cycle; regulation of protein kinase activity; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle

0.05 mL

390 USD