RABBIT ANTI 14-3-3 SIGMA | MyBioSource MBS222334 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI 14-3-3 SIGMA

catalog :

MBS222334

quantity :

0.1 ml

price :

475 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

sheep

application :

western blot

product information

catalog number :

MBS222334

products type :

Antibody

products full name :

RABBIT ANTI 14-3-3 SIGMA

products short name :

14-3-3 SIGMA

other names :

14-3-3 protein sigma; 14-3-3 protein sigma; 14-3-3 protein sigma; Stratifin

products gene name :

14-3-3 SIGMA

other gene names :

SFN; SFN

uniprot entry name :

1433S_SHEEP

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Bovine, Chicken, Human, Mammals (Expected from Sequence), Mouse, Rabbit, Rat

sequence length :

248

specificity :

This item recognises 14-3-3 sigma in all mammals. It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 sigma is expressed mostly in epithelial cells and has been associated with cancers including breast, nasopharyngeal, endometrial adenocarcinoma, prostate, epithelial ovarian, colorectal, adenoid cystic, and papillary. 14-3-3 sigma binds c-fos activating the MAPK pathway. Sequestration of Bax by 14-3-3 sigma inhibits apoptosis whereas sequestration of 14-3-3 sigma by CARP-1 induces it. Mitosis is regulated by 14-3-3 sigma through binding keratin filaments and sequestering cyclin B1 and cdc2. p73-dependent induction of 14-3-3 sigma regulates chemo sensitivity in breast cancers with p53 mutations. In reaction to DNA damage and along with RACK1, 14-3-3 sigma targets, DeltaNp63alpha for proteasome degradation. 14-3-3 also negatively regulates both the glucocorticoid receptor and the activity of PKB/Akt which stabilizes p27 Kip1. This item may not react with recombinant proteins that are not N-acetylated.

form :

Serum. Serum - liquid

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Western Blot (WB)

app notes :

Western Blot: This item detects a band of approximately 30kDa in HEK293 cell lysates. Western Blotting: Maximum Dilution: 1/3000

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide (NaN3)

other info2 :

Immunogen: Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 sigma. Peptide sequence: Ac.MERASLIQKAC. Antiserum Preparation: Antisera to sheep 14-3-3 sigma were raised by repeated immunisations of rabbit with highly purified antigen. Target Species: Sheep

products description :

Rabbit anti 14-3-3 antibody recognizes 14-3-3 sigma in all mammals. It is a member of the 14-3-3 family which consists of 30 kDa proteins involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 is expressed mostly in epithelial cells and has been associated with cancers including breast, nasopharyngeal, endometrial adenocarcinoma, prostate, epithelial ovarian, colorectal, adenoid cystic, and papillary. 14-3-3 binds c-fos activating the MAPK pathway. Sequestration of Bax by 14-3-3 inhibits apoptosis whereas sequestration of 14-3-3 sigma by CARP-1 induces it. Mitosis is regulated by 14-3-3 through binding keratin filaments and sequestering cyclin B1 and cdc2. p73-dependent induction of 14-3-3 sigma regulates chemo sensitivity in breast cancers with p53 mutations. In reaction to DNA damage and along with RACK1, 14-3-3 targets, DeltaNp63alpha for proteasome degradation. 14-3-3 also negatively regulates both the glucocorticoid receptor and the activity of PKB/Akt which stabilizes p27 Kip1. Rabbit anti 14-3-3 antibody may not react with recombinant proteins that are not N-acetylated.

ncbi gi num :

57163961

ncbi acc num :

NP_001009208.1

ncbi gb acc num :

NM_001009208.1

uniprot acc num :

O77642

ncbi mol weight :

27,849 Da

ncbi pathways :

Aldosterone-regulated Sodium Reabsorption Pathway 1030844!!Aldosterone-regulated Sodium Reabsorption Pathway 130590!!Cell Cycle Pathway 1030755!!Cell Cycle Pathway 463!!P53 Signaling Pathway 1030758!!P53 Signaling Pathway 465

size :

0.1 ml

price :

475 USD