RABBIT ANTI 14-3-3 ETA | MyBioSource MBS222331 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI 14-3-3 ETA

catalog :

MBS222331

quantity :

0.1 mL

price :

475 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken, cow

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS222331

products type :

Antibody

products full name :

RABBIT ANTI 14-3-3 ETA

products short name :

14-3-3 ETA

other names :

14-3-3 protein eta; 14-3-3 protein eta; 14-3-3 protein eta; 14-3-3 eta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta; Protein AS1

products gene name :

14-3-3 ETA

other gene names :

YWHAH; YWHAH; YWHA1; YWHA1

uniprot entry name :

1433F_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Bovine, Chicken, Human, Mammals (Expected from Sequence), Mouse, Rabbit, Rat

sequence length :

246

specificity :

This item recognises 14-3-3 eta in all mammals. It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for it's different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 eta is expressed principally in the brain although it is expressed at low levels in other tissues. The protein binds alpha-synuclein, forming part of lewy bodies in parkinsons disease and has been linked to early-onset schizophrenia. The eta isoform binds both gremlin 1, which is overexpressed in human cancers, and the DAL-1/Protein 4.1B tumour suppressor. Furthermore the glucocortcoid receptor is positively regulated by 14-3-3- eta. This item may not react with recombinant proteins that are not N-acetylated.

form :

Serum. Serum - liquid

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Paraffin*, Western Blot (WB)

app notes :

Western Blot: This item detects a band of approximately 30kDa in HEK293 cell lysates. Immunohistology - Paraffin: Maximum Dilution: 1/3200; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. . Western Blotting: Maximum Dilution: 1/3000

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide (NaN3)

other info2 :

Immunogen: Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 eta. Peptide sequence: Ac.GDREQLLQRARC. Histology Positive Control Tissue: Normal brain tissue. Antiserum Preparation: Antisera to sheep 14-3-3 eta were raised by repeated immunisations of rabbit with highly purified antigen. Target Species: Sheep

ncbi gi num :

4507951

ncbi acc num :

NP_003396.1

ncbi gb acc num :

NM_003405.3

ncbi mol weight :

28,219 Da

ncbi pathways :

Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Class I PI3K Signaling Events Mediated By Akt Pathway (138020); Epstein-Barr Virus Infection Pathway (585562)

ncbi summary :

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]

uniprot summary :

14-3-3 eta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Protein type: Nuclear receptor co-regulator; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 22q12.3. Cellular Component: cytoplasmic vesicle membrane; cytoplasm; plasma membrane; cytosol. Molecular Function: protein domain specific binding; insulin-like growth factor receptor binding; protein binding; enzyme binding; sodium channel regulator activity; protein heterodimerization activity; actin binding; glucocorticoid receptor binding. Biological Process: intracellular protein transport; substantia nigra development; apoptosis; regulation of neuron differentiation; glucocorticoid receptor signaling pathway; positive regulation of transcription, DNA-dependent; regulation of synaptic plasticity; glucocorticoid catabolic process; negative regulation of dendrite morphogenesis; regulation of sodium ion transport

size1 :

0.1 mL

price1 :

475 USD

more info or order :

MyBioSource product webpage