catalog number :
MBS222330
products full name :
RABBIT ANTI 14-3-3 EPSILON (C-TERMINAL)
products short name :
14-3-3 EPSILON
other names :
14-3-3 protein epsilon; 14-3-3 protein epsilon; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; 14-3-3 protein epsilon; Protein kinase C inhibitor protein 1; KCIP-1
products gene name :
14-3-3 EPSILON
other gene names :
YWHAE; YWHAE; 14-3-3E; KCIP-1
uniprot entry name :
1433E_SHEEP
reactivity :
Bovine, Chicken, Human, Mammals (Expected from Sequence), Mouse, Rabbit, Rat
specificity :
Rabbit anti 14-3-3 antiubody recognizes a C-terminal epitope of 14-3-3 epsilon, a member of the 14-3-3 family which consists of 30 kDa proteins involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 epsilon is expressed in all mammals. It is deleted in small cell lung cancers disrupting the G checkpoint (Aitken, A. et al. 2006). During apoptosis, cleavage of 14-3-3 protein by caspase-3 increases the affinity between bad and Bcl-x(L). When bound by ERK, 14-3-3 epsilon is thought to deactivate heat shock factor 1 in cells recovering from heat shock. It is also a potential suicide susceptibility gene.
form :
Serum. Serum - liquid
storage stability :
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
ELISA (EIA), Immunohistology Paraffin, Western Blot (WB)
app notes :
Western Blot: Rabbit anti 14-3-3 epsilon antibody detects a band of ~30kDa in HEK293 cell lysates. Western Blotting: Maximum Dilution: 1/3000
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
other info2 :
Immunogen: Synthetic corresponding to C-terminal sequence of sheep 14-3-3 epsilon.Peptide sequence: CGEEQNKEALQDVEDENQ. Histology Positive Control Tissue: Normal brain tissue. Antiserum Preparation: Antisera to sheep anti 14-3-3 epsilon C-terminus were raised by repeated immunisations rabbit of with highly purified antigen. Target Species: Sheep
products description :
Rabbit anti 14-3-3 antiubody recognizes a C-terminal epitope of 14-3-3 epsilon, a member of the 14-3-3 family which consists of 30 kDa proteins involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 epsilon is expressed in all mammals. It is deleted in small cell lung cancers disrupting the G checkpoint (Aitken, A. et al. 2006). During apoptosis, cleavage of 14-3-3 protein by caspase-3 increases the affinity between bad and Bcl-x(L). When bound by ERK, 14-3-3 epsilon is thought to deactivate heat shock factor 1 in cells recovering from heat shock. It is also a potential suicide susceptibility gene.
ncbi mol weight :
29,174 Da
ncbi pathways :
Cell Cycle Pathway 1030755!!Cell Cycle Pathway 463!!Epstein-Barr Virus Infection Pathway 1030886!!Epstein-Barr Virus Infection Pathway 587115!!Hippo Signaling Pathway 1030783!!Hippo Signaling Pathway 750388!!Neurotrophin Signaling Pathway 1030815!!Neurotrophin Signaling Pathway 100064!!Oocyte Meiosis Pathway 1030759!!Oocyte Meiosis Pathway 126872