antibody

RABBIT ANTI HUMAN NF KAPPA B INHIBITOR ALPHA (C-TERMINAL)

MBS222320

0.1 mL

610 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunoprecipitation, enzyme immunoassay

Antibody

RABBIT ANTI HUMAN NF KAPPA B INHIBITOR ALPHA (C-TERMINAL)

NF KAPPA B INHIBITOR ALPHA

NF-kappa-B inhibitor alpha; NF-kappa-B inhibitor alpha; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkappaBalpha; ikB-alpha; major histocompatibility complex enhancer-binding protein MAD3; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3

NFKBIA; NFKBIA; IKBA; MAD-3; NFKBI; IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha

IKBA_HUMAN

Polyclonal

IgG

Rabbit

Mouse, Rat

317

This item is specific for NF-kappa-B inhibitor alpha (also known as IkB alpha), one of seven members of the IkB class of inhibitor proteins that have been identified to date. NFkBIa inhibits the NFkB complex in unstimulated cells through non-covalent binding. Following stimulation of cells, IkB alpha is phosphorylated so that it dissociates from the NFkB, and then ubiquinated prior to degradation.

Serum. Serum - liquid

Total protein concentration 80.0 mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)

Immunohistology - Frozen: Minimum Dilution: 1/1000; Maximum Dilution: 1/5000;. ELISA: Minimum Dilution: 1/20,000; Maximum Dilution: 1/100,000;. Western Blotting: Minimum Dilution: 1/2000; Maximum Dilution: 1/10,000

Perservative Stabilisers: 0.1% Sodium Azide (NaN3)

Immunogen: NFkBIa peptide corresponding to a region near the C-terminus of the human protein, conjugated with KLH. Buffer Solution: Phosphate buffered saline. Target Species: Human

MBS222320 is specific for NF-kappa-B inhibitor alpha (also known as IkB alpha), one of seven members of the IkB class of inhibitor proteins that have been identified to date. NFkBIa inhibits the NFkB complex in unstimulated cells through non-covalent binding. Following stimulation of cells, IkB alpha is phosphorylated so that it dissociates from the NFkB, and then ubiquinated prior to degradation.

10092619

NP_065390.1

NM_020529.2

P25963

35,609 Da

AGE/RAGE Pathway (698754); Activated TLR4 Signalling Pathway (106400); Activation Of NF-kappaB In B Cells Pathway (576251); Adaptive Immune System Pathway (366160); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Apoptosis Pathway (198797); Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Modulation And Signaling Pathway (198822)

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

IkB-alpha: a regulatory protein that inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. Controlled by sequential serine-phosphorylation, ubiquitination and degradation. Protein type: DNA-binding; Inhibitor. Chromosomal Location of Human Ortholog: 14q13. Cellular Component: cytoplasm; plasma membrane; nucleus; cytosol. Molecular Function: identical protein binding; NF-kappaB binding; protein binding; enzyme binding; heat shock protein binding; ubiquitin protein ligase binding; nuclear localization sequence binding; protein complex binding; transcription factor binding. Biological Process: nerve growth factor receptor signaling pathway; viral reproduction; apoptosis; positive regulation of cellular protein metabolic process; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; cytoplasmic sequestering of NF-kappaB; response to exogenous dsRNA; lipopolysaccharide-mediated signaling pathway; positive regulation of interferon type I production; toll-like receptor 4 signaling pathway; cytoplasmic sequestering of transcription factor; negative regulation of myeloid cell differentiation; MyD88-independent toll-like receptor signaling pathway; regulation of NF-kappaB import into nucleus; negative regulation of DNA binding; regulation of cell proliferation; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to muramyl dipeptide; inhibition of NF-kappaB transcription factor; protein import into nucleus, translocation; toll-like receptor signaling pathway; innate immune response; negative regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of apoptosis. Disease: Ectodermal Dysplasia, Anhidrotic, With T-cell Immunodeficiency, Autosomal Dominant

0.1 mL

610 USD