catalog number :
MBS221854
products full name :
RABBIT ANTI UBC13 (C-TERMINAL)
products short name :
UBC13
other names :
ubiquitin-conjugating enzyme E2 N; Ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2 N; bendless-like ubiquitin conjugating enzyme; bendless-like ubiquitin-conjugating enzyme; epididymis secretory protein Li 71; ubiquitin carrier protein N; ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-protein ligase N; yeast UBC13 homolog; ubiquitin-conjugating enzyme E2N; Bendless-like ubiquitin-conjugating enzyme; Ubc13; UbcH13; Ubiquitin carrier protein N; Ubiquitin-protein ligase N
products gene name :
UBC13
other gene names :
UBE2N; UBE2N; UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13; BLU
uniprot entry name :
UBE2N_HUMAN
form :
Purified. Purified IgG - liquid
concentration :
IgG concentration 1.0 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Immunohistology Paraffin*, Western Blot (WB)
app notes :
Western Blot: Rabbit anti human UBC13 antibody detects a band of ~16kDa in human small intestine cell lystates. Immunohistology - Paraffin: Maximum Dilution: 2ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. . Western Blotting: Minimum Dilution: 0.5ug/ml; Maximum Dilution: 1ug/ml
other info1 :
Perservative Stabilisers: 0.02% Sodium Azide
other info2 :
Histology Positive Control Tissue: Mouse thymus tissue. Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to human UBC13 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by ion exchange chromatography. Target Species: Human
products description :
Rabbit anti Human UBC13 antibody recognizes the C-terminal (CT) region of Ubiquitin-conjugating enzyme E2N (UBC13), also known as Bendless-like ubiquitin-conjugating enzyme, Ubiquitin carrier protein N or Ubiquitin-protein ligase N. UBC13 is a ~16kDa enzyme which is an important component of the TLR (Toll-like receptor) and IL-1R signalling pathway. UBC13 is a DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6. Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation (Martein et al. 2009).
ncbi acc num :
NP_003339.1
ncbi gb acc num :
NM_003348.3
ncbi mol weight :
17,138 Da
ncbi pathways :
Activated TLR4 Signalling Pathway 106400!!Adaptive Immune System Pathway 366160!!Antigen Processing: Ubiquitination Proteasome Degradation Pathway 366162!!Antiviral Mechanism By IFN-stimulated Genes Pathway 530760!!Class I MHC Mediated Antigen Processing Presentation Pathway 366161!!Cytokine Signaling In Immune System Pathway 366171!!Downstream TCR Signaling Pathway 106418!!FCERI Mediated NF-kB Activation Pathway 833828!!Fc Epsilon Receptor (FCERI) Signaling Pathway 833825!!IKK Complex Recruitment Mediated By RIP1 Pathway 187090
ncbi summary :
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq, Jul 2008]