SHEEP ANTI HUMAN LOX-1 | MyBioSource MBS221834 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

SHEEP ANTI HUMAN LOX-1

catalog :

MBS221834

quantity :

0.05 mg

price :

440 USD

clonality :

polyclonal

host :

sheep

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry, FACS

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS221834

products type :

Antibody

products full name :

SHEEP ANTI HUMAN LOX-1

products short name :

LOX-1

other names :

oxidized low-density lipoprotein receptor 1 isoform 2; Oxidized low-density lipoprotein receptor 1; oxidized low-density lipoprotein receptor 1; C-type lectin domain family 8 member A; hLOX-1; lectin-type oxidized LDL receptor 1; ox LDL receptor 1; oxidized low-density lipoprotein receptor 1, soluble form; scavenger receptor class E, member 1; oxidized low density lipoprotein (lectin-like) receptor 1; C-type lectin domain family 8 member A; Lectin-like oxidized LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1; Lectin-type oxidized LDL receptor 1Oxidized low-density lipoprotein receptor 1, soluble form

products gene name :

LOX-1

other gene names :

OLR1; OLR1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1; CLEC8A; LOX1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1

uniprot entry name :

OLR1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Sheep

sequence length :

181

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 0.1 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Western Blot: Sheep anti LOX-1 antibody detects intact 50kDa LOX-1 and also a prominent 32kDa proteolytic fragment in western blotting. Immunohistology: For the best results MyBioSource recommend HISTAR detection kits. Flow Cytometry: Maximum Dilution: 1/10. Immunoprecipitation: Maximum Dilution: Neat. Western Blotting: Minimum Dilution: 1/10; Maximum Dilution: 1/100

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. 1% Bovine Serum Albumin

other info2 :

Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to human LOX-1 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography. Target Species: Human

products description :

Sheep anti LOX-1 antibody recognizes the type II membrane glycoprotein LOX-1 (lectin-like oxidized LDL receptor-1), a 40-50kDa class E scavenger receptor, expressed by endothelial cells, macrophages, activated platelets and smooth muscle cells. LOX-1 binds to a wide range of ligands, including oxidized low-density lipoprotein (oxLDL), hypochlorite modified high-density lipoprotein (HDL), aged/apoptotic cells, activated platelets and bacteria, reflecting its versatile physiological functions. MBS221834 will detect LOX-1 on transfected cells and in recombinant protein reconstitution assays. LOX-1 levels are low in normal resting human tissues so may fall below detectable levels. Expression of the LOX-1 gene is upregulated by oxLDL and the binding of LOX-1 to oxLDL results in the activation of NF-kappaB. Furthermore, LOX-1 antibodies have been shown to suppress the oxidized HDL (oxHDL) activation of NF-KappaB in endothelial cells, suggesting that this activation may be due to the binding of oxHDL to LOX-1. Angiotensin II and the inflammatory cytokine Tumour Necrosis Factor alpha (TNFalpha) also invoke an increase in LOX-1 gene expression and studies have focused on its role in endothelial dysfunction (Chen, M. et al.)(Sawamura, T. et al.) and inflammatory diseases such as atherosclerosis (Murphy, J.E. et al.) and rheumatoid arthritis, as well as its affect on CD40/CD40L signaling in both atherosclerosis and human coronary artery endothelial cells (HCAECs) (Li, D. et al.).

ncbi gi num :

290654342

ncbi acc num :

NP_001166103.1

ncbi gb acc num :

NM_001172632.1

uniprot acc num :

P78380

ncbi mol weight :

21,425 Da

ncbi pathways :

Cell Surface Interactions At The Vascular Wall Pathway 106062!!Hemostasis Pathway 106028!!PPAR Signaling Pathway 83042!!PPAR Signaling Pathway 450!!Phagosome Pathway 153910!!Phagosome Pathway 153859

ncbi summary :

This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

size :

0.05 mg

price :

440 USD

more info or order :

MyBioSource product webpage