RABBIT ANTI MEK 1 (pThr386) | MyBioSource MBS221806 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI MEK 1 (pThr386)

catalog :

MBS221806

quantity :

0.1 mL

price :

705 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, chicken, cow

application :

western blot

product information

catalog number :

MBS221806

products type :

Antibody

products full name :

RABBIT ANTI MEK 1 (pThr386)

products short name :

MEK 1

other names :

dual specificity mitogen-activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 1; dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1); mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1

products gene name :

MEK 1

other gene names :

MAP2K1; MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1; MEK1; PRKMK1; MAP kinase kinase 1; MAPKK 1; MKK1; MEK 1

uniprot entry name :

MP2K1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Monkey (Expected from Sequence), Mouse (Expected from Sequence), Rat, Xenopus (Expected from Sequence)

sequence length :

393

specificity :

This item is specific for MEK 1, also known as MAP2K1, when phosphorylated at threonine 386. MEK 1 is an integral component of the MAP kinase cascade that regulates cell growth and differentiation, and may also play a role in brain synaptic plasticity. MEK 1 may be phosphorylated by MAP kinase at Thr292 or Thr38.

form :

Purified. Purified IgG - liquid

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.

tested application :

Western Blot (WB)*

app notes :

Western Blot: This item detects a band of approximately 45kDa in human T47D lysates. Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. 0.01% Bovine Serum Albumin. 50% Glycerol

other info2 :

Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within human MEK 1 which includes phosphorylated Thr386. Buffer Solution: 10mM HEPES pH7.5. Antiserum Preparation: Antisera to phosphorylated MEK 1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. Target Species: Human

products description :

MBS221806 is specific for MEK 1, also known as MAP2K1, when phosphorylated at threonine 386. MEK 1 is an integral component of the MAP kinase cascade that regulates cell growth and differentiation, and may also play a role in brain synaptic plasticity. MEK 1 may be phosphorylated by MAP kinase at Thr292 or Thr386.

ncbi gi num :

5579478

ncbi acc num :

NP_002746.1

ncbi gb acc num :

NM_002755.3

uniprot acc num :

Q02750

ncbi mol weight :

40,764 Da

ncbi pathways :

AGE/RAGE Pathway (698754); ARMS-mediated Activation Pathway (106466); Activated TLR4 Signalling Pathway (106400); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Axon Guidance Pathway (105688); B Cell Receptor Signaling Pathway (198909); B Cell Receptor Signaling Pathway (83081)

ncbi summary :

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

uniprot summary :

MEK1: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf, Mos and Cot kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK1 and ERK2. An essential component of MAP kinase signal transduction pathways involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Protein type: Protein kinase, dual-specificity (non-receptor); EC 2.7.12.2; Protein kinase, STE; Kinase, protein; STE group; STE7 family. Chromosomal Location of Human Ortholog: 15q22.1-q22.33. Cellular Component: dendrite cytoplasm; Golgi apparatus; microtubule; focal adhesion; mitochondrion; endoplasmic reticulum; early endosome; perikaryon; cell cortex; cytosol; axon; perinuclear region of cytoplasm; cytoplasm; late endosome; plasma membrane; microtubule organizing center; nucleus. Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activator activity; protein binding; Ras GTPase binding; receptor signaling protein tyrosine phosphatase activity; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; mitogen-activated protein kinase kinase kinase binding; ATP binding; protein kinase activity. Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; activation of MAPKK activity; protein heterooligomerization; activation of MAPK activity; negative regulation of homotypic cell-cell adhesion; response to glucocorticoid stimulus; cell motility involved in cell locomotion; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; signal transduction; chemotaxis; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; neuron differentiation; negative regulation of cell proliferation; positive regulation of RNA elongation from RNA polymerase II promoter; small GTPase mediated signal transduction; vesicle transport along microtubule; response to axon injury; cell cycle arrest; toll-like receptor 4 signaling pathway; Golgi inheritance; epidermal growth factor receptor signaling pathway; mitosis; fibroblast growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; melanosome transport; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; keratinocyte differentiation; cell proliferation; regulation of stress-activated MAPK cascade; regulation of vascular smooth muscle contraction; Ras protein signal transduction; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; response to oxidative stress; positive regulation of Ras protein signal transduction; positive regulation of cell differentiation; vascular endothelial growth factor receptor signaling pathway; cell motility; positive regulation of cell migration. Disease: Noonan Syndrome 1; Cardiofaciocutaneous Syndrome 3

size1 :

0.1 mL

price1 :

705 USD