antibody

RABBIT ANTI MEK 1/2 (pSER218/222)

MBS221794

0.1 mL

705 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, dog, chicken, cow, zebrafish

Antibody

RABBIT ANTI MEK 1/2 (pSER218/222)

MEK 1/2

dual specificity mitogen-activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 1; dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1); mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1

MEK 1/2

MAP2K1; MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1; MEK1; PRKMK1; MAP kinase kinase 1; MAPKK 1; MKK1; MEK 1

MP2K1_HUMAN

Polyclonal

IgG

Rabbit

Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Monkey (Expected from Sequence), Mouse, Rat (Expected from Sequence), Xenopus (Expected from Sequence), Zebrafish (Expected from Sequence)

393

This item is specific for MEK 1 and MEK 2, (also known as MAP2K1 and MAP2K2 respectively), when phosphorylated at serine 218 and 222. MEK1 and 2 are related dual specificity protein kinases from the MAP kinase family. These proteins are activated by phosphoryation at serine 218 and 222, and play a critical role in mitogen growth factor signal transduction.

Purified. Purified IgG - liquid

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.

Western Blot (WB)*

Western Blot: This item recognises a band of approximately 45kD in serum stimulated mouse NIH 3T3 cell lysates. Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

Perservative Stabilisers: 0.09% Sodium Azide. 0.01% Bovine Serum Albumin. 50% Glycerol

Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence which includes phosphorylated ser 218 and 222 within human MEK1 and 2. Buffer Solution: 10mM HEPES pH7.5. Antiserum Preparation: Antisera to phosphorylated MEK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. Target Species: Human

MBS221794 is specific for MEK 1 and MEK 2, (also known as MAP2K1 and MAP2K2 respectively), when phosphorylated at serine 218 and 222. MEK1 and 2 are related dual specificity protein kinases from the MAP kinase family. These proteins are activated by phosphoryation at serine 218 and 222, and play a critical role in mitogen growth factor signal transduction.

5579478

NP_002746.1

NM_002755.3

40,764 Da

AGE/RAGE Pathway (698754); ARMS-mediated Activation Pathway (106466); Activated TLR4 Signalling Pathway (106400); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Axon Guidance Pathway (105688); B Cell Receptor Signaling Pathway (198909); B Cell Receptor Signaling Pathway (83081)

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

MEK1: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf, Mos and Cot kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK1 and ERK2. An essential component of MAP kinase signal transduction pathways involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, dual-specificity (non-receptor); Protein kinase, STE; STE group; STE7 family. Chromosomal Location of Human Ortholog: 15q22.1-q22.33. Cellular Component: dendrite cytoplasm; Golgi apparatus; microtubule; focal adhesion; mitochondrion; endoplasmic reticulum; early endosome; perikaryon; cell cortex; cytosol; perinuclear region of cytoplasm; axon; late endosome; cytoplasm; plasma membrane; microtubule organizing center; nucleus. Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activator activity; protein binding; Ras GTPase binding; receptor signaling protein tyrosine phosphatase activity; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; mitogen-activated protein kinase kinase kinase binding; ATP binding; protein kinase activity. Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; activation of MAPK activity; protein heterooligomerization; negative regulation of homotypic cell-cell adhesion; response to glucocorticoid stimulus; stress-activated MAPK cascade; cell motility involved in cell locomotion; pathogenesis; toll-like receptor 3 signaling pathway; chemotaxis; signal transduction; toll-like receptor 10 signaling pathway; neuron differentiation; toll-like receptor 5 signaling pathway; negative regulation of cell proliferation; positive regulation of RNA elongation from RNA polymerase II promoter; small GTPase mediated signal transduction; vesicle transport along microtubule; response to axon injury; cell cycle arrest; toll-like receptor 4 signaling pathway; Golgi inheritance; epidermal growth factor receptor signaling pathway; mitosis; fibroblast growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; melanosome transport; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; keratinocyte differentiation; regulation of stress-activated MAPK cascade; cell proliferation; Ras protein signal transduction; regulation of vascular smooth muscle contraction; insulin receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; response to oxidative stress; positive regulation of Ras protein signal transduction; toll-like receptor 9 signaling pathway; positive regulation of cell differentiation; cell motility; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration. Disease: Noonan Syndrome 1; Cardiofaciocutaneous Syndrome 3

0.1 mL

705 USD