catalog number :
MBS221786
products full name :
RABBIT ANTI CREB (pSer133)
products short name :
CREB
other names :
cyclic AMP-responsive element-binding protein 1 isoform B; Cyclic AMP-responsive element-binding protein 1; cyclic AMP-responsive element-binding protein 1; CREB-1; Y protein; cAMP response element binding protein 1; cAMP-responsive element-binding protein 1; cAMP responsive element binding protein 1
products gene name :
CREB
other gene names :
Creb1; Creb1; Creb; Creb-1; CREB-1; cAMP-responsive element-binding protein 1
uniprot entry name :
CREB1_RAT
reactivity :
Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Monkey (Expected from Sequence), Mouse (Expected from Sequence), Sheep (Expected from Sequence), Xenopus (Expected from Sequen
specificity :
This item is specific for rat cyclic AMP response element binding protein (CREB), when phosphorylated at serine 133. CREB is a member of the leucine zipper family of transcription factors, which is involved in a wide range of intracellular signalling processes. Phosphorylation of serine 133 has been demonstrated by several protein kinases, including protein kinase A (PKA), mitogen activated protein kinase (MAPK) and Ca2+ /calmodulin-dependent protein kinase (CaMK). This phophorylation is required for CREB mediated transcription.
form :
Purified. Purified IgG - liquid
storage stability :
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.
tested application :
Western Blot (WB)*
app notes :
Western Blot: This item detects a band of approximately 45kDa in rat hippocampus lysates that had been incubated in the presence of forskolin. Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide. 0.01% Bovine Serum Albumin. 50% Glycerol
other info2 :
Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within CREB which includes phosphorylated Ser133. Buffer Solution: 10mM HEPES pH7.5. Antiserum Preparation: Antisera to phosphorylated rat CREB were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography. Target Species: Rat
products description :
MBS221786 is specific for rat cyclic AMP response element binding protein (CREB), when phosphorylated at serine 133. CREB is a member of the leucine zipper family of transcription factors, which is involved in a wide range of intracellular signalling processes. Phosphorylation of serine 133 has been demonstrated by several protein kinases, including protein kinase A (PKA), mitogen activated protein kinase (MAPK) and Ca2+ /calmodulin-dependent protein kinase (CaMK). This phophorylation is required for CREB mediated transcription.
ncbi acc num :
NP_112279.1
ncbi gb acc num :
NM_031017.1
ncbi mol weight :
35,081 Da
ncbi pathways :
AKT Phosphorylates Targets In The Nucleus Pathway 1120580!!AMPK Signaling Pathway 989832!!AMPK Signaling Pathway 992181!!Activated TLR4 Signalling Pathway 1121566!!Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway 1121188!!Adaptive Immune System Pathway 1121508!!Adipogenesis Pathway 198479!!Adrenergic Signaling In Cardiomyocytes Pathway 908278!!Adrenergic Signaling In Cardiomyocytes Pathway 909696!!Alcoholism Pathway 585585
ncbi summary :
binds the cAMP response element in many gene promoters and regulates transcription [RGD, Feb 2006]