GOAT ANTI HUMAN MCM4 | MyBioSource MBS221742 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

GOAT ANTI HUMAN MCM4

catalog :

MBS221742

quantity :

0.1 mg

price :

625 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot

product information

catalog number :

MBS221742

products type :

Antibody

products full name :

GOAT ANTI HUMAN MCM4

products short name :

MCM4

other names :

DNA replication licensing factor MCM4; DNA replication licensing factor MCM4; DNA replication licensing factor MCM4; CDC21 homolog; homolog of S. pombe cell devision cycle 21; minichromosome maintenance deficient 4; minichromosome maintenance complex component 4; CDC21 homolog; P1-CDC21

products gene name :

MCM4

other gene names :

MCM4; MCM4; NKCD; CDC21; CDC54; NKGCD; hCdc21; P1-CDC21; CDC21

uniprot entry name :

MCM4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Goat

sequence length :

863

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1.0 mg/ml

storage stability :

Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Paraffin*, Western Blot (WB)

app notes :

Western Blot: This item detects a band of approximately 100 kDa in HeLa cell lysates. Immunohistology - Paraffin: Minimum Dilution: 1/200; Maximum Dilution: 1/1000; Application Note: Heat-mediated antigen retrieval is required prior to staining paraffin sections with this antibody. MyBioSource recommend citrate buffer, pH6.0, for this purpose. . Western Blotting: Minimum Dilution: 1/2000; Maximum Dilution: 1/10000

other info1 :

Perservative Stabilisers: 0.1% Sodium Azide (NaN3)

other info2 :

Buffer Solution: TRIS-citrate/Phosphate buffered saline. Antiserum Preparation: Antisera to human MCM4 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography. Target Species: Human

products description :

Goat anti Human MCM4 antibody recognizes DNA replication licensing factor MCM4, also known as human minichromosome maintenance 4 (MCM4) or CDC21 homolog. Human MCM4 is an 863 amino acid, ~95kDa nuclear component of the MCM complex containing a single MCM domain. MCM4 is involved in the initiation and control of eukaryotic DNA replication. Studies show that MCM4 forms a hexameric protein complex with MCM6 and 7, which has DNA helicase activity, and may act as a DNA unwinding enzyme (Kanter et al. 2008). The phosphorylation of MCM4 by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex (Ishimi and Komamura-Kohno 2001).

ncbi gi num :

33469919

ncbi acc num :

NP_005905.2

ncbi gb acc num :

NM_005914.3

uniprot acc num :

P33991

ncbi mol weight :

96,558 Da

ncbi pathways :

Activation Of ATR In Response To Replication Stress Pathway (105754); Activation Of The Pre-replicative Complex Pathway (105777); Assembly Of The Pre-replicative Complex Pathway (105821); C-MYB Transcription Factor Network Pathway (138073); Cell Cycle Pathway (530733); Cell Cycle Checkpoints Pathway (105739); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463)

ncbi summary :

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

uniprot summary :

MCM4: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. The MCM hexameric protein complex is a key component of the pre-replication complex. The complex consisting of this protein and MCM2, 6 and 7 possesses DNA helicase activity. Phosphorylation by CDC2 reduces the DNA helicase activity and chromatin binding of the complex. Protein type: EC 3.6.4.12; DNA replication. Chromosomal Location of Human Ortholog: 8q11.2. Cellular Component: nucleoplasm; membrane; MCM complex; nucleus. Molecular Function: ATP-dependent DNA helicase activity; protein binding; single-stranded DNA binding; ATP binding. Biological Process: DNA unwinding during replication; DNA replication initiation; DNA strand elongation during DNA replication; mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle. Disease: Natural Killer Cell And Glucocorticoid Deficiency With Dna Repair Defect

size1 :

0.1 mg

price1 :

625 USD