GOAT ANTI HUMAN VEGF | MyBioSource MBS221735 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

GOAT ANTI HUMAN VEGF

catalog :

MBS221735

quantity :

0.1 mg

price :

540 USD

clonality :

polyclonal

host :

goat

reactivity :

human

application :

enzyme immunoassay, ELISA, western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS221735

products type :

Antibody

products full name :

GOAT ANTI HUMAN VEGF

products short name :

VEGF

other names :

vascular endothelial growth factor A isoform a; Vascular endothelial growth factor A; vascular endothelial growth factor A; vascular permeability factor; vascular endothelial growth factor A; Vascular permeability factor; VPF

products gene name :

VEGF

other gene names :

VEGFA; VEGFA; VPF; VEGF; MVCD1; VEGF; VEGF-A; VPF

uniprot entry name :

VEGFA_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Goat

sequence length :

412

form :

Purified. Purified IgG - lyophilised

concentration :

IgG concentration 1.0 mg/ml after reconstitution.

storage stability :

Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of reconstitution.

tested application :

ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin*, Western Blot (WB)

app notes :

ELISA: This purified human VEGFantibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human VEGF antibody as the detection reagent and recombinant human VEGF as the standard. Western Blot: This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human VEGF as the positive control. ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml. Functional Assays: Minimum Dilution: 0.05; Maximum Dilution: 0.10ug/ml. Immunohistology - Paraffin: Minimum Dilution: 5.0; Maximum Dilution: 15.0ug/ml; Application Note: Heat-mediated antigen retrieval is required prior to staining paraffin sections with this antibody. Citrate buffer, pH6.0, is recommended for this purpose. . Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml

other info1 :

Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage, the addition of 0.09% soidum azide is recommended. NB: For functional studies do not add azide.

other info2 :

Histology Positive Control Tissue: Human breast invasive ductal carcinoma. Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to human VEGF were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography. Target Species: Human

products description :

Goat anti Human VEGF antibody specifically recognizes human VEGF (vascular endothelial growth factor), a heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which exists as several splice variants of the VEGF gene. VEGF, originally named vascular permeability factor due to its ability to act as vasodilator and increase microvascular permeability, is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis. The functional forms of VEGF exist as disulphide-linked homodimers and are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VMBS23026165. All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), whilst the closely-related VEGF-C and VEGF-D, can also bind to VEGFR-3 (Flt-4).

ncbi gi num :

76781480

ncbi acc num :

NP_001020537.2

ncbi gb acc num :

NM_001025366.2

uniprot acc num :

P15692

ncbi mol weight :

34,406 Da

ncbi pathways :

Angiogenesis Pathway 198772!!Axon Guidance Pathway 105688!!Bladder Cancer Pathway 83115!!Bladder Cancer Pathway 527!!Cellular Response To Hypoxia Pathway 645259!!Cellular Responses To Stress Pathway 645258!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Developmental Biology Pathway 477129!!EPH-Ephrin Signaling Pathway 1127691

ncbi summary :

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]

size :

0.1 mg

price :

540 USD

more info or order :

MyBioSource product webpage