RABBIT ANTI HUMAN SDF-1 ALPHA | MyBioSource MBS221732 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI HUMAN SDF-1 ALPHA

catalog :

MBS221732

quantity :

0.1 mg

price :

540 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

ELISA, western blot, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS221732

products type :

Antibody

products full name :

RABBIT ANTI HUMAN SDF-1 ALPHA

products short name :

SDF-1 ALPHA

other names :

stromal cell-derived factor 1 isoform beta; Stromal cell-derived factor 1; stromal cell-derived factor 1; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor; chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH; Pre-B cell growth-stimulating factor; PBSF

products gene name :

SDF-1 ALPHA

other gene names :

CXCL12; CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12; SDF1; SDF1A; SDF1B; SDF-1; hSDF-1; IRH; hIRH; PBSF

uniprot entry name :

SDF1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

sequence length :

form :

Purified. Purified IgG - lyophilised

concentration :

IgG concentration 1.0 mg/ml after reconstitution.

storage stability :

Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of reconstitution.

tested application :

ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin*, Western Blot (WB)

app notes :

ELISA: This purified human SDF-1alpha antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human SDF-1alpha antibody as the detection reagent and recombinant human SDF-1alpha as the standard . Western Blot: This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human SDF-1alpha as the positive control. ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml. Functional Assays: Minimum Dilution: 2.0; Maximum Dilution: 4.0ug/ml. Immunohistology - Paraffin: Minimum Dilution: 0.5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. . Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml

other info1 :

Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.

other info2 :

Histology Positive Control Tissue: Human colorectal adenocarcinoma. Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to human SDF-1 alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. Target Species: Human

products description :

Rabbit anti Human SDF-1 alpha antibody recognizes human SDF-1 alpha, otherwise known as CXCL12a, a 68 amino acid stromal cell derived CXC chemokine, which arises from alternative splicing of the SDF-1 gene. The two isoforms, SDF-1 alpha and SDF-1 beta, share an identical amino acid sequence, except for an additional four residues in the C-Terminal region of the beta isoform. SDF-1 binds with high-affinity to the G protein -coupled receptor CXCR4 (fusin) and acts as a chemoattractant for T and B lymphocytes, monocytes and migratory neurons, and is a vital factor in haematopoiesis and angiogenesis. By competitively binding to CXCR4, SDF-1 acts as an inhibitor for the CXCR4-mediated entry of HIV-1 virus into target T-cells, and the SDF-1-CXCR4 interaction is important for the regulation of trafficking of normal and malignant stem cells.

ncbi gi num :

10834988

ncbi acc num :

NP_000600.1

ncbi gb acc num :

NM_000609.6

uniprot acc num :

P48061

ncbi mol weight :

11,004 Da

ncbi pathways :

Axon Guidance Pathway 83065!!Axon Guidance Pathway 476!!CXCR4-mediated Signaling Events Pathway 137910!!Chemokine Receptors Bind Chemokines Pathway 106359!!Chemokine Signaling Pathway 99051!!Chemokine Signaling Pathway 96864!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway 1127664

ncbi summary :

This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

size :

0.1 mg

price :

540 USD

more info or order :

MyBioSource product webpage