RABBIT ANTI HUMAN INTERLEUKIN-8 | MyBioSource MBS221719 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI HUMAN INTERLEUKIN-8

catalog :

MBS221719

quantity :

0.1 mg

price :

540 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS221719

products type :

Antibody

products full name :

RABBIT ANTI HUMAN INTERLEUKIN-8

products short name :

IL-8

other names :

interleukin-8; Interleukin-8; interleukin-8; T-cell chemotactic factor; alveolar macrophage chemotactic factor I; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; emoctakin; granulocyte chemotactic protein 1; interleukin 8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; neutrophil-activating peptide 1; small inducible cytokine subfamily B, member 8; tumor necrosis factor-induced gene 1; chemokine (C-X-C motif) ligand 8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factorCleaved into the following 7 chains:MDNCF-aAlternative name(s):GCP/IL-8 protein IV; IL8/NAP1 form I

other gene names :

CXCL8; CXCL8; IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1; IL8; IL-8; GCP-1; MDNCF; MONAP; NAP-1; LYNAP; NAF

uniprot entry name :

IL8_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

sequence length :

form :

Purified. Purified IgG - lyophilised

concentration :

IgG concentration 1.0 mg/ml "after reconstitution

storage stability :

Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of reconstitution.

tested application :

ELISA (EIA), Functional Assays (FN), Immunofluorescence (IF), Immunohistology Paraffin*, Western Blot (WB)

app notes :

ELISA: This purified human IL-8 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human IL-8 antibody as the detection reagent and recombinant human IL-8 as the standard. Western Blot: This purified human IL-8 antibody may used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human IL-8 as the positive control. ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml. Functional Assays: Minimum Dilution: 2.0; Maximum Dilution: 3.0ug/ml. Immunohistology - Paraffin: Minimum Dilution: 0.25; Maximum Dilution: 1.0ug/ml; Application Note: This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose. . Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml

other info1 :

Reconstitution: Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide.

other info2 :

Immunogen: Recombinant human IL-8. Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to human IL-8 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. Target Species: Human

products description :

Rabbit anti Human Interleukin-8 antibody recognizes human IL-8 (Interleukin-8), a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-Terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1(NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.

ncbi gi num :

10834978

ncbi acc num :

NP_000575.1

ncbi gb acc num :

NM_000584.3

uniprot acc num :

P10145

ncbi mol weight :

11,338 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); ATF4 Activates Genes Pathway (530772); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chagas Disease (American Trypanosomiasis) Pathway (147809)

ncbi summary :

The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]

uniprot summary :

IL8: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. Belongs to the intercrine alpha (chemokine CxC) family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q13-q21. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; chemokine activity; interleukin-8 receptor binding. Biological Process: regulation of cell adhesion; neutrophil chemotaxis; neutrophil activation; negative regulation of G-protein coupled receptor protein signaling pathway; unfolded protein response; calcium-mediated signaling; chemotaxis; signal transduction; regulation of retroviral genome replication; induction of positive chemotaxis; negative regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; response to molecule of bacterial origin; receptor internalization; immune response; angiogenesis; inflammatory response; cell motility; cell cycle arrest; embryonic gut development

size1 :

0.1 mg

price1 :

540 USD

more info or order :

MyBioSource product webpage