RABBIT ANTI BCL-2 BINDING COMPONENT 3 | MyBioSource MBS221697 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI BCL-2 BINDING COMPONENT 3

catalog :

MBS221697

quantity :

0.1 mg

price :

365 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS221697

products type :

Antibody

products full name :

RABBIT ANTI BCL-2 BINDING COMPONENT 3

products short name :

BCL-2 BINDING COMPONENT 3

other names :

bcl-2-binding component 3 isoform 1; Bcl-2-binding component 3; bcl-2-binding component 3; p53 up-regulated modulator of apoptosis; BCL2 binding component 3; JFY-1; p53 up-regulated modulator of apoptosis

products gene name :

BCL-2

other gene names :

BBC3; BBC3; JFY1; PUMA; JFY-1; PUMA

uniprot entry name :

BBC3_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse (Expected from Sequence)

sequence length :

261

specificity :

This item recognises the p53 - upregulated modulator of apoptosis (PUMA), also known as Bcl-2 binding component 3. Bcl-2 binding component 3 is a pro-apoptotic member of the BCL-2 family. Bcl-2 binding component 3 is localised to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53.

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1.0 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Paraffin*, Western Blot (WB)

app notes :

Western Blot: This item detects a band of approximately 23kDa in K562 cell lysates. Immunohistology - Paraffin: Maximum Dilution: 10 ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. . Western Blotting: Minimum Dilution: 2ug/ml; Maximum Dilution: 4ug/ml

other info1 :

Perservative Stabilisers: 0.02% Sodium Azide

other info2 :

Immunogen: Synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human Bcl-2 binding component 3. Histology Positive Control Tissue: Human breast carcinoma. Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to human Bcl-2 binding component 3 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. Target Species: Human

products description :

MBS221697 recognises the p53 - upregulated modulator of apoptosis (PUMA), also known as Bcl-2 binding component 3. Bcl-2 binding component 3 is a pro-apoptotic member of the BCL-2 family. Bcl-2 binding component 3 is localised to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53.

ncbi gi num :

187829730

ncbi acc num :

NP_001120712.1

ncbi gb acc num :

NM_001127240.2

uniprot acc num :

Q9BXH1

ncbi mol weight :

10,326 Da

ncbi pathways :

Activation Of BH3-only Proteins Pathway (105658); Activation Of PUMA And Translocation To Mitochondria Pathway (105661); Apoptosis Pathway (198797); Apoptosis Pathway (105648); Apoptosis Modulation And Signaling Pathway (198822); BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway (105664); DNA Damage Response Pathway (198788); DNA Damage Response (only ATM Dependent) Pathway (198827); Direct P53 Effectors Pathway (137939); Hippo Signaling Pathway (749777)

ncbi summary :

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

uniprot summary :

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Mitochondrial. Chromosomal Location of Human Ortholog: 19q13.3-q13.4. Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol. Molecular Function: protein binding. Biological Process: caspase activation; release of cytochrome c from mitochondria; apoptosis; positive regulation of protein homooligomerization; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; reduction of endoplasmic reticulum calcium ion concentration; determination of adult life span; response to DNA damage stimulus; negative regulation of growth

size1 :

0.1 mg

price1 :

365 USD

more info or order :

MyBioSource product webpage