MyBioSource

antibody

RABBIT ANTI AIF (aa517-531)

MBS221629

0.1 mg

385 USD

polyclonal

rabbit

mouse, human, rat

western blot

MBS221629

Antibody

RABBIT ANTI AIF (aa517-531)

AIF

apoptosis-inducing factor 1, mitochondrial isoform 4; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor 1, mitochondrial; Neuropathy, axonal motor-sensory, with deafness and mental retardation; neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome); programmed cell death 8 (apoptosis-inducing factor); striatal apoptosis-inducing factor; apoptosis-inducing factor, mitochondrion-associated, 1; Programmed cell death protein 8

AIF

AIFM1; AIFM1; AIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6; AIF; PDCD8

AIFM1_HUMAN

Polyclonal

IgG

Rabbit

Mouse, Rat

261

Purified. Purified IgG - liquid

IgG concentration 0.5 mg/ml

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Paraffin*, Western Blot (WB)

Immunohistology - Paraffin: Maximum Dilution: 10 ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. . Western Blotting: Minimum Dilution: 0.25ug/ml; Maximum Dilution: 1ug/ml

Perservative Stabilisers: 0.02% Sodium Azide

Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to AIF were raised by repeated immunisation of rabbit with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography. Target Species: Human

Rabbit anti Human AIF antibody recognizes human Apoptosis-inducing factor 1, mitochondrial, also known as Programmed cell death protein 8 or apoptosis inducing factor (AIF). AIF is a 613 amino acid member of the FAD-dependent oxidoreductase family.Rabbit anti Human AIF antibody reacts with an internal region of AIF (within the region aa 517-531. AIF is widely expressed in human tissues and localizes in the mitochondria. AIF translocates to the nucleus upon induction of apoptosis. Multiple isoforms of AIF are generated by alternative splicing. Rabbit anti Human AIF antibody recognizes isoforms 1, 2,3 and 5. It is not expected to recognize either isoforms 4 or 6 as they lack the immunogen sequence. Mutations in the AIFM1 can result in various disease conditions including COXPD6 and Cowchock syndrome.

195927004

NP_001124318.1

NM_001130846.2

O95831

26,033 Da

Apoptosis Pathway 83060!!Apoptosis Pathway 470!!Apoptosis Modulation By HSP70 Pathway 198877!!Ceramide Signaling Pathway 138023

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, May 2010]

0.1 mg

385 USD