RABBIT ANTI HUMAN ZIP KINASE (aa279-298) | MyBioSource MBS221597 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI HUMAN ZIP KINASE (aa279-298)

catalog :

MBS221597

quantity :

0.1 mg

price :

320 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

product information

catalog number :

MBS221597

products type :

Antibody

products full name :

RABBIT ANTI HUMAN ZIP KINASE (aa279-298)

products short name :

ZIP KINASE

other names :

death-associated protein kinase 3; Death-associated protein kinase 3; death-associated protein kinase 3; DAP kinase 3; DAP-like kinase; MYPT1 kinase; ZIP-kinase; zipper-interacting protein kinase; death-associated protein kinase 3; DAP-like kinase; Dlk; MYPT1 kinase; Zipper-interacting protein kinase; ZIP-kinase

products gene name :

ZIP

other gene names :

DAPK3; DAPK3; DLK; ZIP; ZIPK; ZIPK; DAP kinase 3; Dlk; ZIP-kinase

uniprot entry name :

DAPK3_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

sequence length :

454

specificity :

ZIP kinase is a serine/threonine kinase that has high sequence homology to DAP kinase (death associated protein kinase), a known mediator of apoptosis.This item recognises ZIP kinase in Western blots, detecting a band of 52kD.

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 0.5mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Frozen, Western Blot (WB)

app notes :

Western Blotting: Maximum Dilution: 1/1000

other info1 :

Perservative Stabilisers: 0.02% Sodium Azide

other info2 :

Immunogen: Synthetic peptide corresponding to amino acids 279-298 of human ZIP kinase. Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to human ZIP kinase were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. Target Species: Human

products description :

ZIP kinase is a serine/threonine kinase that has high sequence homology to DAP kinase (death associated protein kinase), a known mediator of apoptosis. MBS221597 recognises ZIP kinase in Western blots, detecting a band of 52kD.

ncbi gi num :

4557511

ncbi acc num :

NP_001339.1

ncbi gb acc num :

NM_001348.2

uniprot acc num :

O43293

ncbi mol weight :

37,048 Da

ncbi pathways :

Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Extrinsic Pathway (105649); Pathways In Cancer (83105); Programmed Cell Death Pathway (1127510); Role Of DCC In Regulating Apoptosis Pathway (161011)

ncbi summary :

Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008]

uniprot summary :

DAPK3: Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, actin cytoskeleton reorganization, cell motility, smooth muscle contraction, and mitosis, particularly cytokinesis. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase- dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Regulates myosin phosphorylation in both smooth muscle and non- muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A, and the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates STAT3 and enhances its transcriptional activity. Positively regulates the canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Can disrupt the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates histone H3 on Thr-11 at centromeres during mitosis. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. Monomer and homotrimer. Can also exist as homodimer or form heterodimers with ATF4. Homodimerization is required for activation segment autophosphorylation Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. According to PubMed:17953487, does not interact with PARW. Interacts with AATF, CDC5L, UBE2D1, UBE2D2 AND UBE2D3. Interacts with AR and this interaction is enhanced by AATF. Interacts (via leucine zipper) with TCP10L (via leucine zipper). Interacts (via kinase domain) with DAPK1 (via kinase domain).Interacts with STAT3, NLK and TCF7L2. Isoform 1 and isoform 2 can interact with myosin and PPP1R12A. Isoform 2 is expressed in the bladder smooth muscle. Inhibited by pyridone 6 (K00225), a potent, ATP-competitive inhibitor. Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. Oligomerization is required for full enzymatic activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Tumor suppressor; CAMK group; DAPK family. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: PML body; cytoplasm; nucleus; lipid raft. Molecular Function: protein serine/threonine kinase activity; identical protein binding; leucine zipper domain binding; protein binding; Rho GTPase binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; cAMP response element binding protein binding; ATP binding. Biological Process: regulation of smooth muscle contraction; transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; regulation of mitotic cell cycle; regulation of myosin II filament assembly or disassembly; cytokinesis; chromatin modification; protein amino acid phosphorylation; neuron differentiation; regulation of apoptosis; regulation of cell shape; regulation of transcription, DNA-dependent; negative regulation of translation; regulation of focal adhesion formation; regulation of mitosis; regulation of autophagy; positive regulation of cell migration

size1 :

0.1 mg

price1 :

320 USD